骨髓间充质干细胞-生物蛋白胶复合物修复兔膝关节软骨缺损  

Repair of knee articular cartilage defects with bone marrow-derived mesenchymal stem cells and bioprotein gel compound in rabbits

作　　者：李新江[1] 方艳伟[1] 张爱民[1] 赵建辉[1] 

机构地区：[1]河北医科大学第二医院急诊外科,石家庄050000

出　　处：《中华创伤杂志》2015年第6期563-567,共5页Chinese Journal of Trauma

基　　金：全军医药卫生科研基金资助项目（082002）

摘　　要：目的探讨骨髓间充质干细胞（BMSCs）与生物蛋白胶（Bg）的复合物对兔膝关节软骨缺损的修复作用。方法将36只健康新西兰大耳白兔制作成直径3．5mm、深3mm双侧股骨髁关节软骨缺损模型，按随机数字表法分为三组：BMSCs—Bg组（植入BMSCs—Bg复合物）、Bg对照组（植入Bg）、空白对照组（不植入任何材料），每组12只。术后4，8，12，16周分别行大体解剖学、HE染色观察软骨修复情况，根据关节软骨组织学半定量评分标准进行评分。结果BMSCs～Bg组4周即形成类透明软骨组织，8，12周接近正常关节软骨，16周修复软骨仍未退变；而两对照组以成纤维组织及纤维软骨修复为主。术后4，8，12，16周BMSCs～Bg组组织学评分分别为（4．5±0．1）分、（4．9±0．1）分、（5．5±0．1）分、（6．2±0．1）分，均高于同时相点两对照组（P〈0．05）；Bg对照组评分均高于空白对照组，但差异均无统计学意义。结论BMSCs与Bg的复合物在短期内能够良好的修复关节软骨全层缺损，Bg是良好的细胞载体，有助于软骨的形成。Objective To investigate the role of bone marrow-derived mesenchymal stem cells （BMSCs） compounded with bioprotein gel （Bg） in repair of knee articular cartilage defects of rabbits. Methods Bilateral femoral condylar cartilage defect models （ 3.5 mm in diameter and 3 mm in depth） were established in 36 healthy New Zealand rabbits. Animals were divided into three groups of 12 rabbits each according to the random number table： BMSCs-Bg group, Bg group and blank control group. Effect of repair was evaluated by anatomic observation and histological examination at 4, 8, 12 and 16 weeks after operation and by articular cartilage histological hemi-quantitative scoring system. Results Hyaline-like cartilage was noted in BMSCs-Bg group at 4 weeks postoperatively, got close to the normal at 8 and 12 weeks and showed no disintegration at 16 weeks. Only fibrous tissue or fibrocartilage was observed in other 2 groups. In BMSCs-Bg group histology scores were （4.5 ± 0.1 ）points, （4.9± 0. 1 ） points, （5.5 ±0. 1 ） points, and （6.2 ± 0.1 ） points respectively at 4, 8, 12 and 16 weeks, higher than those in other 2 groups （ P 〈 0.05 ）. Besides, the histology scores were higher in Bg group than in blank control group but the differences were insignificant. Conclusion BMSCs-Bg compound can well repair the articular cartilage defect in a short-term period, when Bg contributes to cartilage generation as a good cell carrier.

关 键 词：软骨 间质干细胞 纤维蛋白组织粘着剂 修复 

分 类 号：R684[医药卫生—骨科学]