藏药红景天甙诱导DC成熟并增强T细胞抑制肺癌的实验研究  

SRR promotes maturation of dendritic cells whereby enhances suppression of lung cancer by T cells-an experimental study

作　　者：贺拥军[1] 岳秋娟[1] 张敏[1] 

机构地区：[1]西藏民族学院医学院,西藏自治区生命科学基础实验室,咸阳712082

出　　处：《现代免疫学》2015年第3期226-229,共4页Current Immunology

基　　金：西藏民族学院院科研重大项目培育计划(15MYZP02)

摘　　要：观察红景天甙对Lewis肺癌移植瘤生长的作用,初步探讨其增强肿瘤免疫的机制。建立小鼠Lewis肺癌移植瘤模型,随机分为红景天甙(sachalin rhodiola rhizome,SRR)组,紫杉醇(taxelol,TAX)组和PBS组,计算抑瘤率并观察小鼠生存期;同时取SRR治疗荷瘤小鼠肿瘤引流DC,观察SRR体外处理后对DC表面分子、吞噬功能及T细胞杀伤功能的影响。在治疗第23天,SRR组肿瘤体积明显小于PBS组(P<0.05),与TAX组无显著性差异(P>0.05);SRR组小鼠生存期较PBS组显著延长(P<0.05),与TAX组无显著差异(P>0.05);SRR处理的树突细胞(dendritic cell,DC)表面分子MHCII、CD80表达显著上升,吞噬功能显著下降,由DC诱导的活化T细胞对肿瘤细胞的杀伤效应显著高于对照组(P<0.05)。SRR有效促进DC成熟、增强T细胞功能,同时可以抑制肿瘤生长。To observe the effect of sachalin rhodiola rhizome （SRR） on Lewis lung transplantation tumor and primarily explore the underline mechanism by which SRR suppresses tumor growth. C57BL/6 tumor-bearing mice were established and anticancer experiments were conducted on these mice after being randomly divided into 3 groups： SRR group, taxelol （TAX） group and PBS group. The changes of tumor volume were recorded, the tumor inhibition rates were calculated and the survival time was observed. DCs were isolated and the influence of SRR on the expression of surface markers, on the phagocytosis and the capability of inducing cytotoxic T cells were evaluated, on day 23 after treatment with SRR, surface MHCII and CD80 were upregulated whereas the ability to pbagocytose was abated. Compared with the control group, DC of the SRR group elicited more potent tumoricidal activity of T cells. Tumor volume of the SRR group was significantly smaller than that of the PBS group（P 〈0.05）, but showed no significant difference when compared with PTX group （P〉0.05）. Compared to PBS group, the survival time of SRR group was significantly prolonged（P〈0.05）, but showed no significant difference when compared with PTX group （P〉0.05）. Thus, SRR significantly promotes maturation of DC,enhances CTL function and improve anti-tumor effect.

关 键 词：红景天甙 肺癌 DC T细胞 

分 类 号：R392[医药卫生—免疫学]