蛋白质合成抑制剂对萌发玉米超弱光子辐射的影响  

Effect of protein synthesis inhibitors on ultraweak photon emission during germination of corn

作　　者：习岗[1] 赵燕燕[1] 刘锴[1] 贺瑞瑞[1] 

机构地区：[1]西安理工大学理学院应用物理系,西安710048

出　　处：《农业工程学报》2015年第10期303-308,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金(31471412;51277151);西安理工大学科技创新计划项目(2013CX019)

摘　　要：为了揭示种子萌发过程中超弱光子辐射机理,分别采用蛋白质合成的转录抑制剂放线菌素D(actinomyin D,AMD)和翻译抑制剂环己亚胺酮(cycloheximide,CHM)处理萌发玉米种子,研究了玉米萌发过程中鲜质量的变化以及自发光子辐射和外界光诱导的延迟光子辐射的变化。结果表明,50μg/m L的AMD部分抑制了萌发玉米鲜质量的增长,100μg/m L的CHM完全抑制了萌发玉米鲜质量的增长,萌发玉米自发光子辐射强度的增长与种子鲜质量的增长呈现正相关(相关系数r分别为0.95492、0.93218和0.96235)。研究还发现,在玉米萌发过程中,AMD和CHM对延迟光子辐射的增长有不同的抑制作用,CHM部分抑制了延迟光子辐射的初始光子数、相干时间和积分强度的增大,AMD则使初始光子数、相干时间和积分强度不再增加。研究结果为揭示种子萌发过程中超弱光子辐射的机理及其技术开发提供参考。Ultraweak photon emission in germination of plant seeds which includes spontaneous photon emission and delayed photon emission induced by external light is a kind of life information. Spontaneous photon emission is closely related to important life processes such as DNA synthesis, cell division, respiratory metabolism and biological oxidation. Delayed photon emission is related to functional status of biological systems. The study of ultraweak photon emission in germination of plant seeds has very important significance in the mechanism of seed germination, resistance evaluation, identification of germplasm and so on. In order to reveal the ultraweak photon emissions mechanism in seed germination process, the transcription inhibitor of protein synthesis i.e. actinomyin D （AMD） and translation inhibitor i.e. cycloheximide （CHM） were used to treat germinating corn seed respectively. The changes of the fresh quality, spontaneous photon emission and delayed photon emission of germinating corn were studied in this paper. The results showed that the 50 μg/mL AMD partly inhibited the increase of fresh quality during the germination of corn, 100 μg/mL CHM completely inhibited the increase of fresh quality of germinating corn, and there was a positive correlation between the spontaneous photon emission and seed’s fresh quality during the germination of corn （correlation coefficient was 0.96235）. It was implied that the intensity of spontaneous photon emission from germinating corn could be used as a signal for indicating the germinating status of corn. The study also found that AMD and CHM had different inhibitory effects for the increase of delayed photon emission during germination of corn, of which, the inhibition from AMD was stronger than CHM. It was speculated that respiratory metabolism and RNA/DNA synthesis in germinating corn could be the sources of spontaneous photon emission. The further study on delayed photon emission induced by external light found that the initial photon numberI0and coherenc

关 键 词：辐射 种子 机理 萌发玉米 转录抑制剂 翻译抑制剂 细胞超弱光子辐射 呼吸代谢 RNA/DNA合成 

分 类 号：O482.31[理学—固体物理] Q632[理学—物理]