黄曲霉毒素B_1对雏鸡外周血T淋巴细胞亚群及血清IL–2和IFN–γ的影响  

Effects of aflatoxin B_1 on the decrease of peripheral blood T–lymphocyte subsets and serum interleukin–2 or interferon–γ in broilers

作　　者：于正强[1] 陈瑾[1,2] 彭西[1] 方静[1] 陈科杰[1] 何杨[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]西昌市畜牧局,四川西昌615000

出　　处：《湖南农业大学学报（自然科学版）》2015年第3期294-298,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：教育部长江学者和创新团队发展计划项目(IRT 0848);四川省科学技术厅项目(2013FZ0072)

摘　　要：为探明黄曲霉毒素B1(AFB1)对雏鸡细胞免疫功能的影响,将100只1日龄艾维茵健康公雏鸡随机分为4组,分别喂以基础日粮(玉米–豆粕型基础日粮,对照)和AFB1日粮(基础日粮中AFB1的添加量分别为0.15、0.3、0.6 mg/kg,分别记为AFB1Ⅰ、Ⅱ、Ⅲ组),试验期21 d。以流式细胞术检测雏鸡外周血T淋巴细胞亚群变化;用酶联免疫吸附试验(ELISA)检测雏鸡血清IL–2和IFN–γ含量变化。结果显示,与对照组相比,AFB1Ⅱ和AFB1Ⅲ组7、14和21日龄雏鸡外周血CD3+,CD3+CD4+和CD3+CD8+T淋巴细胞百分比下降明显(P<0.05或P<0.01),血清中IL–2和IFN–γ含量降低(P<0.05或P<0.01),AFB1Ⅰ组雏鸡14日龄的血清IL–2和7日龄的IFN–γ含量降低(P<0.05),表明摄食含黄曲霉毒素B1的日粮可导致雏鸡细胞免疫功能下降。The changes of T–lymphocytes subsets around peripheral blood were detected employing flow cytometer and the content of serum IL–2 and IFN–γ were determined using ELISA method to judge the damage of cellular immune function induced by AFB1 exposure based on one hundred male avian broilers fed with a control diet and three AFB1 diets（with 0.15, 0.3, 0.6 mg/kg amount of AFB1 addition, respectively, marked with AFB1Ⅰ,Ⅱ, Ⅲ in turn）for 21-day. The results showed that the percentage of CD3^＋, CD3^＋CD4^＋ and CD3^＋CD8^＋ T lymphocytes were decreased from 7 to 21 days of age（P〈0.05 or P0.01）in AFB1Ⅱ and AFB1 Ⅲ groups, and the contents of serum IL–2 at 14 days of age and IFN–γ at 7 days of age were decreased in AFB1 Ⅰ group（P0.05）. The results indicated that the cellular immune function was declined by consumption the diet of AFB1 contamination.

关 键 词：雏鸡 黄曲霉毒素B1 T淋巴细胞亚群 IL–2 IFN–γ 流式细胞术 

分 类 号：S851.347.2[农业科学—预防兽医学]