耐盐Bacillus sp.9BS的筛选及其对染料的脱色效果  

Screening of a salt-tolerant Bacillus sp.9BS and its decoloring effect on dye

作　　者：汪春蕾[1] 田菲[1] 张月颖 孙鹤敏 李启育 张凯鑫[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《湖南农业大学学报（自然科学版）》2015年第3期313-317,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：中央高校基本科研业务费专项基金(DL12CA08);东北林业大学生命科学学院大学生创新训练项目

摘　　要：利用含铜的富集培养基,从东北林业大学实验林场白桦林下的土壤中筛选出1株菌株,研究其生理生化特性,结合经典形态学及16S rDNA序列同源性分析,鉴定该菌株为芽孢杆菌属细菌,并将其命名为Bacillus sp.9BS。菌株9BS最适生长温度为37℃,最适生长p H值为7.0,并具有很好的耐盐性,在7%Na Cl溶液中生长良好。以丁香醛连氮为底物,以芽孢干重计量,其芽孢漆酶的活性高达47.1 U/g。菌株9BS的芽孢漆酶在不含介体的情况下,对靛红(IC)和结晶紫(CV)的脱色效果较为显著,6 h脱色率分别为73.3%和82.2%。Enrichment medium added with copper ions was used to screen bacteria possessing laccase activity from birch forest soil. A bacterium with high laccase activity was identified as Bacillus sp. 9BS by morphological, physiological and biochemical characteristics with 16 S rDNA sequence analysis. The optimum growth temperature of strain 9BS was 37 ℃and the optimum growth p H was 7.0. The strain 9BS had the resistance to salt and grew well in 7% Na Cl solution. Using syringaldazine as substrate, the laccase activity of the dry spore of Bacillus sp. 9BS was 47.1 U/g. In the absence of mediator, the spore laccase of strain 9BS showed effective decolorization effect on Isatin（IC） and crystal violet（CV）, the decolorization rates in 6 h were 73.3% and 82.2%, respectively.

关 键 词：细菌漆酶 芽孢杆菌属 染料脱色 

分 类 号：S154.34[农业科学—土壤学] X592[农业科学—农业基础科学]