不同施氮期对小麦千粒重影响的QTL分析  

QTL Mapping Analysis on the Effect of Various Nitrogen Supplying Dates on Thousand Grain Weight in Wheat

作　　者：崔勇[1] 刘小芳[1] 张新业[1] 朱占玲[1] 田宾[1] 田纪春[1] 

机构地区：[1]山东农业大学作物生物学国家重点实验室小麦品质育种研究室,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2015年第3期331-336,共6页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：国家转基因重大专项(2011ZX08002-003);国家自然科学基金项目(31171554)

摘　　要：本研究以花培3号×豫麦57的168个双单倍体（doubled haploid,DH）群体为材料,根据2年12个环境下千粒重性状的表型数据和含有323个位点的分子遗传图谱,对千粒重性状进行了QTL分析。结果共检测到40个QTL,主要集中在染色体2D、3A、4D、5B、6A和7D上。其中,2010年莱阳点3种施氮期下检测到8个QTL,2010年泰安试验点共检测到13个QTL,2011年济源点检测到12个QTL,2011年泰安试验点检测到7个QTL,单个QTL所解释的表型变异介于4.19%～23.14%之间。亲本花培3号对于千粒重的贡献占主导地位。Qtgw3A-2、Qtgw5B、Qtgw6A-1和Qtgw7D-1在三个施氮期都检测到,说明这些QTL是氮肥对千粒重影响较大的QTL。Qtgw3A-2、Qtgw4D、Qtgw6A-1、Qtgw7D-1等在多个试验点均能检测到,说明这些QTL是稳定表达的QTL。总之,影响千粒重的QTL数目及其QTL表达效应在不同施氮期下有很大的变化,说明不同施氮期对千粒重基因的表达存在特异性。小麦育种中,上述结果可为今后合理追施氮肥,增加粒重和产量及千粒重的分子标记辅助选择提供理论依据。In this study, the QTLs/genes related to thousand grain weight were studied in twelve different environments for two years, using a doubled haploid（DH） population derived from a cross between two elite Chinese wheat cultivars Huapei3×Yumai57 for QTL mapping of thousand grain weight based on unconditional quantitative trait locus（QTL） analyses.A total of 40 unconditional QTLs were detected, including 8 QTL detected in Laiyang and 13 QTL detected in Taian in 2010,12 QTL detected in Jiyuan and 7 QTL detected in Taian in 2011. Most QTLs detected in this study are located on chromosome 2D, 3A, 4D, 5B, 6A, and 7D. The phenotypic variance explained by single QTL varied from 4.19% to 23.14%.The effects were mostly contributed by the parent Huapei3. Of the QTLs, Qtgw3A-2, Qtgw5 B, Qtgw6A-1 and Qtgw7D-1were detected under three nitrogen supplying date, indicating that these QTL related to thousand grain weight were affected by nitrogen. Qtgw3A-2, Qtgw4 D, Qtgw6A-1 and Qtgw7D-1 were detected at various experimental plots, suggesting that these QTL were expressed stably. Therefore, the number and the expression of QTLs have a great change under different nitrogen supplying dates, which suggest that the QTLs were specifically expressed in wheat under different nitrogen application stage.The findings in this study should be useful for topdressing nitrogen reasonably and manipulating the QTLs by marker assisted selection（MAS） and be potential in increasing grain yield and grain weight.

关 键 词：施氮期 小麦 千粒重 数量性状位点 

分 类 号：S512.1[农业科学—作物学]