荧光免疫层析试条光电信号处理及特征量选取  

Fluorescent immune-chromatographic strip signal processing and feature selection

作　　者：高跃明[1,2] 李天麒[1,2] 林传阳 潘少恒[2,3] 韦孟宇[2,3] 杜民[1,2] 

机构地区：[1]福州大学物理与信息工程学院,福州350108 [2]福州大学福建省医疗器械和医药技术重点实验室,福州350002 [3]澳门大学科技学院电机及电脑工程系,澳门特别行政区999078

出　　处：《电子测量与仪器学报》2015年第5期662-668,共7页Journal of Electronic Measurement and Instrumentation

基　　金：科技部台港澳合作(2012DFM30040);福建省产学合作重大(2011Y4007)资助项目

摘　　要：荧光免疫层析定量检测技术是一种正在兴起的即时检验技术。在前期荧光免疫层析定量检测仪的研究基础上,采用小波算法对A/D采样后的荧光信号进行去噪处理,选用中值滤波剔除基线漂移,最后比较了采用测试线峰值和面积作为特征量的定量检测效果。数据显示,去噪后信噪比提高10 d B,相对均方差减小30.4%;采用峰值和面积值作为定量特征量的测量CV值分别为5.1%和0.4%,测量误差分别为9.1%和0.9%。结果表明采用积分面积作为特征量对荧光信号进行定量检测具有更好的准确度和重复性。Immunofluorescence chromatographic quantitative detection technology is an emerging technique of the point of care test （POCT）. Based on the previous work of immunofluorescence quantitative chromatography detector, the wavelet algorithm was used to denoise the fluorescence signal after the A/D sampling in this paper. Then the baseline drift was removed by the median filter method. Finally, the peak height and the area were sellected as the characteristic value for the quantitative detection. The experimental data shown that the SNR improved 10dB and the SRMS reduced by 30.4% after denoising. In the quantitatively detect step, the CV were 5.1% and 0.4% , and the test error were 9.1% and 0.9% , for the peak hight and area characteristic value, respectively. The results show that the peak area of the test line as the characteristic value has better accuracy and repeatability than the peak height.

关 键 词：免疫层析 定量检测 特征量 基线漂移 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]