MPG1基因驱动GFP基因在大丽轮枝菌中表达  

The MPG1 Gene Act as Promoter to Drive GFP Gene to Express in Verticillium dahlia

作　　者：张斯[1] 柳燕[1] 徐婷婷[1] 吕乐[1] 竺锡武[2] 陈集双[3] 

机构地区：[1]浙江理工大学生物工程研究所,杭州310018 [2]湖南人文科技学院生命科学系,湖南娄底417000 [3]南京工业大学生物资源工程研究所,南京210009

出　　处：《科技通报》2015年第5期92-96,共5页Bulletin of Science and Technology

基　　金：国家自然科学基金(31071729)资助

摘　　要：为了探讨以MPG1基因作为启动子能否驱动GFP基因在大丽轮枝菌中表达,采用根癌农杆菌介导的真菌遗传转化方法,将含有MPG1-eGFP表达盒的pHMG载体转化入棉花大丽轮枝菌T-9中。经抗生素筛选后转化子约80个/106孢子,荧光显微镜观察转化子菌株孢子和菌丝在荧光显微镜下均发出绿色荧光,基因组DNA-PCR检测、GFP蛋白westernblot检测表明,GFP蛋白在大丽轮枝菌转化子中成功表达。结果表明,MPG1基因作为启动子成功驱动GFP基因在大丽轮枝菌中转录表达。To study whether the MPG1 gene were used as the promoter to drive GFP gene to express in Verticillium dahlia, via Agrobacterium tumefaciens-mediated transformation the pHMG vector with MPG1-eGFP gene were transformed into spores of the Verticillium dahlia T-9. There were 80 transformants each 106 spores. The green fluorescence was found from the spores and hypha of the transformants by the Fluorescence Microscope. By the genomic DNA-PCR and western blot, the results also suggested that the GFP gene were expressed in Verticillium dahlia. All the results showed that the MPG1 gene could act as a promoter to drive GFP gene to expression in Verticillium dahlia.

关 键 词：pHMG 大丽轮枝菌 ATMT GFP MPG1 

分 类 号：S188[农业科学—农业基础科学]