血清和卵泡液中睾酮水平与体外受精-胚胎移植结局的关系  

Levels of Testosterone in Serum and Follicular Fluid Associated with Clinical Outcomes of IVF-ET

作　　者：田东梅[1] 庄静[1] 任洁[1] 张霞[1] 朱明辉[1] 

机构地区：[1]成都中医药大学第二附属医院生殖中心,610041

出　　处：《国际生殖健康／计划生育杂志》2015年第3期203-205,共3页Journal of International Reproductive Health/Family Planning

基　　金：四川省科技厅科技支撑计划项目(2011FZ0019)

摘　　要：目的：探讨体外受精-胚胎移植（IVF-ET）治疗时血清和卵泡液中睾酮浓度与IVF-ET结局的关系。方法：选择2011年5—8月在成都中医药大学第二附属医院生殖中心接受IVF-ET或胞浆内单精子注射（ICSI）-ET治疗的患者43例,检测患者基础血清及控制性超促排卵（COH）时血清和卵泡液中睾酮水平。1按获卵数分3组,低反应（L）组（1-5个）、正常反应（M）组（6-15个）、高反应（H）组（〉15个）,分析各组血清和卵泡液中睾酮浓度及各组受精率和妊娠率。2按临床妊娠情况分为妊娠组与未妊娠组,比较2组间睾酮的差异。结果：1不同获卵数3组间卵泡液中睾酮水平和基础睾酮水平差异无统计学意义（P〉0.05）;人绒毛膜促性腺激素（h CG）日3组血清睾酮水平比较差异有统计学意义（P〈0.05）,其中H组高于M组及L组（P分别为0.010和0.001）;COH中其余时间点睾酮水平差异无统计学意义（P〉0.05）。23组受精率、优质胚胎率差异无统计学意义（P〉0.05）;临床妊娠率比较差异有统计学意义（P〈0.05）。3妊娠组各阶段血清睾酮水平仅在取卵日时高于未妊娠组,差异有统计学意义（P〈0.05）;2组卵泡液中睾酮差异无统计学意义（P〉0.05）。结论：IVF-ET治疗时基础血清睾酮水平不能预测卵巢反应和妊娠结局,但h CG日睾酮水平可能预测卵巢反应;取卵日血清睾酮与妊娠有关;卵泡液中睾酮对妊娠结局无显著影响。Objective： To explore the relationship between the testosterone （T） levels in serum and follicular fluid and the outcomes of stimulation in vitro fertilization-embryo transfer （IVF-ET） cycle. Methods： Data was collected from 43 patients who received IVF/intracytoplasmic sperm injection （ICSI） treatment between May and August in 2011 at our center. T levels in serum and follicular fluid were detected by chemiluminiscence ELISA. ①43 patients were divided into 3 groups by the number of harvested oocytes： group L （1-5 oocytes）, group M （6-15 oocytes） and group H （〉 15 oocytes）. T levels, the fertilization rate and the clinical pregnancy rate were compared among three groups. ②43 patients were divided into the pregnant group and the non-pregnant group. T levels were then compared. Results： ①There were not significant differences in the follicular fluid T levels and the basal serum T level among three groups（P〉0.05）. There were significant differences in the serum T levels on the day of hCG administration among three groups （P 〈0.05）. T level on the day of hCG administration in the H group was significantly higher than that in the M group （P=0.010） and the L group （P= 0.001）. However, there were not significant differences in the serum T levels at other time points of COH among three groups （P〉0.05）. ②There were not significant differences in the fertilization rate and the rate of high quality embryo among three groups （P〉0.05）, however there was significant difference in the clinical pregnancy rate （P〈0.05）. ③The serum T level on the day of ovum pick-up in the pregnant group was significantly higher than that in the non-pregnant group （P〈0.05）; however there was not significant difference in the T level of follicular fluid （P〉0.05）. Conclusions： During the IVF-ET treatment, the basal level of serum T can not predict ovarian response and pregnancy outcome; however the T level on the day of hCG administration could be a param

关 键 词：受精 体外 胚胎移植 睾酮 超排卵 妊娠结局 卵泡液 

分 类 号：R714.8[医药卫生—妇产科学]