机构地区:[1]佳木斯大学康复医学院儿童神经康复实验室,黑龙江佳木斯154002
出 处:《中华临床医师杂志(电子版)》2015年第9期104-107,共4页Chinese Journal of Clinicians(Electronic Edition)
基 金:佳木斯大学研究生科技创新项目(LM2014_48)
摘 要:目的探讨不同强度运动干预对宫内感染致脑损伤仔鼠运动功能的影响。方法对孕鼠腹腔注射脂多糖(LPS)以制备脑损伤仔鼠模型,随机选取对照组(A组)足月仔鼠30只,LPS组足月仔鼠90只并将其随机分为三组:低强度干预组(T1组),中等强度干预组(T2组),大强度干预组(T3组),每组各30只。所有仔鼠出生第二天给予早期抚触直至15日龄,15日龄对各组仔鼠采用悬吊试验和改良BBB运动功能评定对其进行运动功能评定。15日龄干预组仔鼠开始进行运动干预,25日龄对各组仔鼠进行神经行为学评定,检测出脑瘫鼠并继续干预至45日龄,运动干预项目分别为恒温游泳、跳台训练、跑笼训练,按照强度大小各组训练时间为每天5 min、10 min、15 min。运动干预30 d后,对各组仔鼠进行运动功能评定。结果 (1)25日龄仔鼠经神经行为学检测A组无脑瘫鼠,T1组3只脑瘫鼠,T2组2只脑瘫鼠,T3组无脑瘫鼠;(2)15日龄仔鼠运动干预组T1组与T2组,T1组与T3组,T2组与T3组仔鼠悬吊试验评分和改良BBB运动功能评分均无统计学差异(P>0.05);45日龄仔鼠干预组T1组与T2组悬吊试验和改良BBB运动功能评分无统计学差异(P>0.05),T3组与T1组、T3组与T2组均有统计学差异(P<0.01);(3)T1、T2、T3组仔鼠训练前后15 d和45 d悬吊试验和改良BBB运动功能评分均有统计学差异(P<0.01)。结论 (1)早期运动干预可明显改善宫内感染致脑损伤仔鼠运动功能。(2)不同干预强度对脑损伤仔鼠运动功能改善程度不一,较大干预强度对脑损伤仔鼠运动功能改善较大。Objective To explore the effects of different intensity intervention on motor function on brain injured rats. Methods Intraperitoneal injection of LPS was used to make brain injured model. 30 full-term neonatal rats were randomly selected as normal control group. 90 full-term neonatal rats from LPS group were randomly selected as intervention group. In 2 days after birth, touching and massage were used for all the neonatal rats until 15 days. Then took suspend test and modified BBB motor function test for the rats. Chose 30 rats from intervention group as low intensity group (T1 group) , another 30 rats as middle intensity group (T2 group) ant the last 30 rats as high intensity group (T3 group). Each group will receive motor intervention to 45 days. The motor intervention included swimming jumping and running. According to the intervention intensity, each group will train for 5 min, 15 min, 25 min one day. After 30 days intervention, gave each group rats motor function assessment. Results (1)25 days later after behavioral neuroscience test, 3 cerebral palsy rats were identified in T1 group, 2 in T2 group, none in A and T3 group. (2)After 15 days, the score of suspending test and modified BBB motor function assessment did not have statistical significance between T1 and T2, T1 and T3, T2 and T3 group , while after 45 days the score had changed in T1 and T2 group without statistical significance (P〉0.05) , but T1 and T3 , T2 and T3 group had statistical significance (P〈0.01). (2)After intervention, group T3 got remarkable higher score in suspending test and modified BBB motor function assessment. Conclusion (1) Motor intervention can develop the motor function of brain injured rats induced by Intraperitoneal injection of LPS. (2) Different intensity can get different outcomes on motor function on brain injured rats and high intensity intervention can greatly improve the motor function of brain injured rats.
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