电针联合嗅鞘细胞移植对大鼠脊髓损伤神经轴突再生及走向的影响  

Effect of electro-acupuncture combined with olfactory ensheathing cell transplantation on spinal cord injury axonal regeneration and direction

作　　者：谭龙旺[1] 秦兆邦[1] 朱峰[1] 杨利学[1] 

机构地区：[1]陕西中医学院附属医院骨伤科,陕西咸阳712000

出　　处：《中国骨伤》2015年第5期441-445,共5页China Journal of Orthopaedics and Traumatology

基　　金：陕西省教育厅专项科研项目(编号:2010JK508)~~

摘　　要：目的 :探讨电针对嗅鞘细胞(OECs)移植大鼠轴突再生的影响及作用机制。方法:2.5月龄Sprague Dawley(SD)雄性大鼠72只,体重(220±20)g,采用挫伤加全横断的造模方法造成T9脊髓损伤模型后随机分成模型组、电针组、嗅鞘细胞组和电针加嗅鞘细胞组。各组动物于造模后4周和8周注射5%荧光金水溶液(flurosecentgold,FG)0.5μl进行逆行标记,后进行荧光金逆行示踪观察以及动物行为学观察(BBB评分)。结果:(1)BBB评分结果示术后第1天至第1周,各组间比较差异无统计学意义(P>0.05);从第3周开始,电针+OECs组高于模型组、OECs组、电针组3组(P<0.05)。(2)荧光金逆行示踪结果显示:术后4、8周各组脊髓内均可观察到有FG阳性神经纤维再生;在脊髓损伤区,电针加OECs组通过脊髓损伤区荧光金标记的阳性神经纤维数量多于其他3组,走行较其他3组规则。结论:电针联合OECs移植治疗能够极大的促进脊髓损伤大鼠神经纤维的再生以及大鼠后肢功能的恢复,能够恢复神经传导通路,并对再生的神经纤维生长方法有一定的导向性。Objective：To explore the impact and mechanism of electro acupuncture（EA）on olfactory ensheathing cells （OECs）transplantation of spinal cord injury（SCI）axonal regeneration. Methods：In the experiment,72 adult Sprague Daw-ley male rats weighted（220＆#177;20）g underwent contusion and transection method to cause the T9 model of spinal cord injury, were randomly divided into four groups involving model group,EA group,OECs group,and EA＋OECs group. 5%fluorescein gold（FG）solution of 0.5μl was injected into rats＆#39; spinal cord at 4 weeks and 8 weeks after SCI,a series of tests were per-formed including fluorescein gold（FG）retrograde tagging,BBB scores. Results：（1）The BBB scores level among four groups had no differences from the 1st day to the 1st week after the SCI（P〉0.05）. From the 3rd week after the SCI,the BBB scores level in EA＋OECs group were obviously higher than that of other groups（P〈0.05）.（2）The result of the fluorescein gold （FG）retrograde tagging showed at 4 weeks and 8 weeks after treatment FG positive nerve fibers were observed in SCI region. In EA＋OECs group the number of FG positive nerve fibers was more than other three groups,and the fibers were more regular-ly arranged than other three groups. Conclusion：The combination of electro acupuncture and OECs transplantation can recov-er the pathway of nerve conduction and promote nerve fibers regeneration and hind limb function recovery for SCI rat ,and can guide the trend of the axonal regeneration.

关 键 词：脊髓损伤 电针 嗅鞘细胞 神经轴突再生 

分 类 号：R651.3[医药卫生—外科学]