梅毒螺旋体传代接种对兔梅毒模型RPR滴度的影响  

Effect of Treponema pallidum continuous passage inoculation in rabbits model on RPR titer

作　　者：于瑞星[1] 尹跃平[1] 韩燕[1] 钟铭英[1] 施美琴[1] 王琪[1] 龚匡隆[1] 

机构地区：[1]中国医学科学院皮肤病研究所,南京210042

出　　处：《中国麻风皮肤病杂志》2015年第5期273-275,共3页China Journal of Leprosy and Skin Diseases

基　　金：国家十一五"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项"防治性病对预防艾滋病的作用研究"(编号:2008ZX10001-005)

摘　　要：目的:明确兔睾丸组织中Tp Nichols菌株传代接种对实验兔血清学滴度的影响。方法:按照接种Tp菌株代数的不同,将23只家兔分为原代组(5只)、一代组(9只)、二代组(8只),空白对照组(1只)。原代组所接种菌株为保存于液氮的Tp Nichols复苏菌株;一代组实验兔所接种菌株来自于原代组实验兔肿大睾丸;二代组实验兔所接种菌株来自于一代组实验兔肿大睾丸。接种14天后抽取兔耳缘静脉血并空气注射致死,对兔的耳缘静脉血清进行RPR和TPPA检测。结果:接种后实验兔全部感染。原代组、一代、二代组RPR滴度≥1:16的兔比例分别为20%,88.9%,100%,原代组与一代、二代组比较差异均有统计学意义(P=0.008;P=0.001),一代组与二代组比较,差异无统计学差异(P=0.249)。RPR几何平均滴度在原代组、一代组、二代组分别为4.0,25.4,76.1,原代组与一代组、二代组间比较,差异有统计学差异(P<0.05),一代与二代组间比较差异无统计学差异(P>0.05)。结论:在建立梅毒兔实验模型时,将液氮中保存的Tp Nichols菌株在睾丸组织中传代接种,可提高实验兔的梅毒血清学滴度。Objective： To determine the effect of Treponema pallidum （Tp） of continuous passage inocula- tion in rabbits on RPR titer. Methods： Twenty three New Zealand male rabbits were divided into the primary group （5 rabbits）, the first generation group （9 rabbits）, the second generation group （8 rabbits） and the blank control group （ I rabbit）. In the primary group, the testicles of rabbits were inoculated with Nichols strains stored in liquid nitrogen container. In the first generation group, the testicles of rabbits were inoculated with the strains from the rabbits of the primary generation group. In the second generation group, the testicles of rabbits were inoculated with the strains from the rabbits of the first generation group. The levels of RPR and TPPA in blood sera from ear margin veins were detected after 14 days of the inoculation. Results ： All the in- oculated rabbits were infected by Tp. The rates of the rabbits with RPR titer more than or equal to 1 ： 16 in the primary group, the first generation group and the second generation group were 20%, 88.9% and 100%. And the RPR geometrical mean in the primary group, the first generation group and the second generation group were 4.0, 25.4 and 76.1. Conclusion： The serum titers of Tp increased in rabbits of continuous passage inoc- ulation.

关 键 词：梅毒螺旋体 新西兰雄兔 感染模型 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]