己烯雌酚对幼年香猪睾丸与附睾雌激素受体和一氧化氮合酶表达和分布的影响  

Effects of diethylstilbestrol on the expression of estrogen receptor and endothelial nitric oxide synthase in testis and epididymis of male Xiang pigs

作　　者：钟垒[1] 主性[1] 冯杰[1] 田兴贵[1] 

机构地区：[1]贵州大学动物科学学院,贵阳550025

出　　处：《解剖学报》2015年第3期373-378,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(31260591)

摘　　要：目的探讨己烯雌酚(DES)对幼年香猪睾丸和附睾中雌激素受体(ERs)和内皮型一氧化氮合酶(e NOS)表达的影响,及DES对诱导雄性动物生精异常的作用机制。方法将20只20~25日龄幼年雄性香猪分成4组,对照组只注射生理盐水,实验组经腹腔每天注射DES(实验1组:0.03mg/kg;实验2组:0.3mg/kg;实验3组:3mg/kg),连续注射9d。最后1次注射24h后手术取出左侧睾丸和附睾组织经中性多聚甲醛固定,石蜡包埋,常规制备5μm石蜡切片,采用免疫组织化学SP法检测睾丸和附睾组织中ERα、ERβ和e NOS的表达。结果 ERα只在输出小管上皮细胞胞核中表达;ERβ在输出小管上皮细胞、间质细胞及附睾管上皮细胞胞核中均有表达,在睾丸生精小管细胞胞质中也有少量表达。DES能够使ERα在输出小管中的表达率显著下降(P<0.05)。随着DES用量的增多,ERβ在输出小管和附睾管中的表达率显著升高(P<0.05),当DES用量为3mg/kg时ERβ在输出小管和附睾管中的表达率明显高于用量0.03mg/kg和0.3mg/kg(P<0.05)。但0.03mg/kg用量的DES却使睾丸生精小管中ERβ的表达率显著性下降(P<0.05),并在0.3mg/kg和3mg/kg用量时在睾丸生精小管中未能检测到ERβ受体的表达。e NOS在睾丸生精小管细胞和输出小管上皮细胞中都有表达,DES能促进e NOS在睾丸生精小管细胞中表达(P<0.05)而降低其在输出小管细胞中表达(P<0.05)。结论 DES明显影响ERs和e NOS在睾丸和附睾中的表达。Objective To investigate the effect of diethylstilbestrol （DES） on the expression of estrogen receptors and endothelial nitric oxide synthase in the testis and epididymis of male Xiang pigs. Methods Twenty（20-25-days-old） pigs were randomly divided into four groups,one control group and three treatment groups, with 5 pigs in each group. The animals in 3 treatment groups were intraperitoneally injected diethystilbestrol at doses of 0.03 mg/（ kg- d） , 0. 3mg/（ kg. d）, 3mg/（kg-d）,respectively for 9 days. The left testes of each animal was excised 24 hours after last injection. The testicular and epididymal tissues were sectioned at a thickness of 5t^m for immunohistochemical staining. The immunohistochemistry technique was used to observe estrogen receptors, including ERa and ERI3, and endothelial nitric oxide synthetase distribution. Results The immunohistochemical positive staining for ERct was only distributed in the nuclei of the efferent ductule epithelia and the ERI3 was distributed in the nuclei of the epithelia of both the efferent ductule and epididymis tube. The ER[3 was distributed in the cytoplasm of the seminiferous tubule. The percentage of ERa positive cells in the efferent ductule was significantly decreased, whereas is ER[3 positive cells in the efferent ductule and epididymis tube were increased （P 〈 0.05） after exposure to DES, but the percentage of ERJ3-positive cells of testis seminiferous tubule significantly decreased at 0.03mg/kg dose of DES （P 〈 0.05 ） , and no ER[3 expression in testes seminiferous tubule at 0. 3 mg/kg, 3 mg/kg dose of DES. The eNOS immunoreactivity was distributed in the seminiferous tubule and efferent ductule epithelia. The percentage of eNOS positive cells in seminiferous tubule was significantlyincreased （P 〈0.05）. In the contrast, eNOS positive cells in efferent ductule epithelia were decreased（P 〈 0. 05 ） after exposure to DES. Conclusion DES has a significant effect on the expression of ERs and eNOS in testis.

关 键 词：己烯雌酚 睾丸 附睾 雌激素受体 上皮型一氧化氮合酶 免疫组织化学 香猪 

分 类 号：S852.1[农业科学—基础兽医学]