山丹黄参多糖对四氯化碳诱导的慢性肝损伤的保护作用  

Protective effects of Shandan Sphallerocarpus gracilis polysaccharide on chronic hepatic injury in mice induced by CCl_4

作　　者：袁静[1] 俞诗源[1,2] 孟茹[1] 魏娉婷[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]西北师范大学学报编辑部,兰州730070

出　　处：《解剖学报》2015年第3期387-393,共7页Acta Anatomica Sinica

基　　金：甘肃省自然科学基金资助项目(1107RJZA141);兰州市社会发展基金资助项目(2013-3-72;2014-1-105)

摘　　要：目的探讨山丹黄参多糖对四氯化碳（CCl4）小鼠肝损伤的保护作用。方法将40只小鼠分为正常对照组、实验对照组、山丹黄参多糖1~3组5个组,山丹黄参多糖1~3组分别连续灌胃浓度为12.5、25.0和37.5g/L的山丹黄参多糖（共28 d,每天2次,每次0.2ml）,正常对照组和实验对照组灌胃等量的生理盐水,灌胃多糖第3天起,实验对照组：山丹黄参多糖1、2、3组各组小鼠于下午灌胃2 h后分别腹腔注射0.2%CCl4橄榄油溶液0.2ml（每3天1次,共9次）,正常对照组腹腔注射等量的生理盐水。用光学显微镜观察肝组织的结构变化,用比色法检测血浆谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）和肝组织超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量的变化,用免疫组织化学法检测Caspase-3及Bax蛋白在肝组织表达的变化。结果实验对照组血浆ALT、AST和ALP含量显著升高（P〈0.01）,肝组织SOD活力明显降低（P〈0.01）,MDA含量显著升高（P〈0.01）,肝组织结构不清,肝细胞炎性坏死,空泡化严重,Caspase-3蛋白和Bax蛋白阳性表达明显增强（P〈0.01）。山丹黄参多糖各组血浆ALT、AST、ALP活性明显降低,肝组织SOD活力显著升高（P〈0.01）,MDA含量明显下降（P〈0.01）,肝索清晰,炎性坏死减少,肝细胞结构清晰,Caspase-3及Bax蛋白阳性表达明显减少（P〈0.01）。结论山丹黄参多糖可提高细胞抗氧化活性,具有抗炎、抗氧化、抑制细胞凋亡和保护肝细胞等作用,能有效减轻CCl4对肝组织的损伤,且在一定范围内呈剂量效应。Objective To investigate the effect of Shandan Sphallerocarpus gracilis polysaccharide（SGP） on the hepatic injury in mice induced by carbon tetrachloride（ CC14 ）. Methods Forty mice were divided into 5 groups ： the nature control group,the experimental control group,and 3 SGP groups. The mice in SGP groups were administered with SGP at the doses of 12.5,25,37.5 g/L 0. 2ml,respectively, the other groups were garaged with 0.2ml saline, the nature control group was infected by 0.2ml injected by 0. 2ml saline. The morphological changes for hepatic tissues were observed under microscopy, the activities of plasma alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, alkaline phosphatase（ALP） and superoxide dismutase （SOD）, as well as content of malondialdehyde （MDA） in liver were determined by colorimetry, the expression change of Caspase-3 and Bax in liver tissue was determined by the immunohistochemical method. Results The activities of plasma ALT, AST, ALP was significantly increased in the experimental control group, compared with the nature control group （P 〈 0. 01 ）, while the activity of SOD in liver tissue decreased obviously （P 〈 0.01 ） , and the contents of M DA were increased significantly （P 〈 0. 01 ） , the livers tissue structure in experimental control group was blur,liver ceils were necrosis and had serious vacuolation. The activities for Caspase-3 and Bax immunohistochemical positive staining in hepatic tissues significantly increased in the experimental control group （P 〈0. 01 ）. However, the activities of plasma ALT, AST and ALP in the SGP groups was significantly decreased （P 〈 O. 01） , the activitys of SOD in liver tissue were increased significantly （ P 〈 0. 01 ）, and the contents of MDA were significantly decreased（P 〈 0. O1 ） , the liver structures were normal, inflammatory necrosis were decreased, liver celluarstuctures were clear,the positive expression of Caspase-3 and Bax protein were decreased significantly,

关 键 词：山丹黄参多糖 四氯化碳 肝脏 组织结构 超氧化物歧化酶 比色法 小鼠 

分 类 号：R338.21[医药卫生—人体生理学]