陈皮中微生物的分离与鉴定  

Isolation and Characterization of Microbes from Pericarpium Citri Reticulatae

作　　者：阳洁[1] 尹坤[1] 谭习羽 袁涛[1] 江院[1] 谭志远[1] 

机构地区：[1]华南农业大学农学院,广东广州510642 [2]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《微生物学报》2015年第6期700-706,共7页Acta Microbiologica Sinica

基　　金：国家自然科学基金项目(31370052);广东省科技项目(2012B010300018;2013B060400020)~~

摘　　要：【目的】陈皮保存的时间越长即陈皮越老其药用功效越显著。本工作拟从7年陈皮中分离有益微生物,并制成相应的微生物产品,应用于高品质耐储存陈皮的生产。【方法】以7年陈皮为材料,用4种培养基分离微生物;采用SDS-PAGE全细胞蛋白电泳和IS-PCR指纹图谱对所分离到的微生物进行聚类分析,再选取每个类群的代表菌株进行分子系统发育分析及生理生化鉴定。【结果】从7年陈皮中分离、纯化23株细菌,但没有分离到真菌与放线菌。23株细菌聚为4个类群,其中类群I属于芽孢杆菌属(Bacillus),类群II、类群III、类群IV属于类芽孢杆菌属(Paenibacillus)。【结论】4个类群中只有类群II代表菌株cp20具有明显的柠檬酸盐利用能力,在陈皮的试验制作过程中具有防腐耐储存作用。[Objective]The longer the Pericarpium Citri Reticulatae is preserved,the better medicinal values will be.The present work aims to isolate beneficial microbes isolated from the 7-year orange peel that might be used to produce high quality Pericarpium Citri Reticulatae.[Methods]The microbes isolated from the 7-year orange peel using 4 mediums were grouped by SDS-PAGE patterns of the whole-cell protein electrophoresis and IS-PCRDNA fingerprinting.The representative strains were further studied by physiological and biochemical tests,and phylogeny analysis.[Results]Total 23 bacteria were obtained from the 7-year orange peel.These strains were classified into 4 groups： the strains of group I belonged to Bacillus; the strains of group II,group III and group IV were closed to Paenibacillus.[Conclusion]Among the representative strains of group I,II,III and IV,only the representative strain cp20 of group II has the ability of strong utilizing citrate obviously.The strain cp20 of group II were further applied to making Pericarpium Citri Reticulatae.

关 键 词：陈皮 芽孢杆菌属 类芽孢杆菌属 

分 类 号：Q93[生物学—微生物学]