17β-雌二醇对脂肪细胞内脂滴增殖的抑制作用及其机制研究  

Negative effect of 17β-E2 on lipid droplets through CIDEc and Plin1 in primary human adipocyte

作　　者：邓婷[1] 颜玲[1] 

机构地区：[1]中山大学附属第三医院整形美容科,广州510630

出　　处：《中国临床解剖学杂志》2015年第3期320-324,共5页Chinese Journal of Clinical Anatomy

摘　　要：目的探讨雌激素对h ASCs成脂分化过程中脂滴相关特异基因DNA断裂因子相似蛋白C(The cell death-inducing DNA fragmentation 45-like effector,CIDEc)、脂滴包被蛋白(Perilipin1,Plin1)m RNA表达的影响。方法对3例患者行脂肪抽吸术,并进行h ASCs培养及传代,鉴定表面标志物;经典鸡尾酒法诱导其成脂,不同浓度(10-8、10-7、10-6mol/L)17β-雌二醇(17β-E2)作用于细胞,分别于24 h、4、7、11d收获细胞,用RT-PCR检测CIDEc、Plin1 m RNA表达情况。结果各组细胞经药物作用后,脂肪细胞中CIDEc及Plin1 m RNA的表达水平随着17β-E2浓度的升高而下降;而随着作用时间的延长,CIDEc及Plin1 m RNA表达水平分别于7 d和4 d达到高峰期,其后逐渐下降。当17β-E2浓度为10-6mol/L时,细胞中CIDEc及Plin1 m RNA的表达水平最低,差异有统计学意义(P<0.01);而加入雌激素受体阻滞剂ICI182780的一组细胞,雌激素对CIDEc及Plin1 m RNA表达的抑制作用明显减弱。结论 17β-E2能够显著降低脂肪细胞分化过程中CIDEc及Plin1的m RNA表达,并可能通过这一途径抑制脂肪细胞内脂滴的增殖。Objective To evaluate the effect of different concentrations of 17β-estradiol（17β-E2）on adipocytes lipid droplet growth and CIDEc, Plin1 mRNA expression in vitro. Methods Human primary adipose tissue-derived stem cells（hASCs） were isolated from liposuction aspirates of three patients and cultured, passaged and identified the cell surface markers. hASCs were induced differentiated at 0 mol/L（17β-E2 free DMEM/F12）,10-8 mol/L, 10-7 mol/L and 10-6 mol/L 17β-E2 and ICI182780 was added to culture human primary preadipocytes. The expression of CIDEc and Plin1 mRNA was detected by RT-PCR after staining with oil red O. Result Different concentrations of 17β-E2 suppressed CIDEc mRNA and Plin1 mRNA expression in human primary preadipocytes. The expression peak of CIDEc and Plin1 mRNA occurred at 7 and 4 days. At 10-6 mo/L 17β-E2,the expression of CIDEc and Plin1 mRNA were the lowest. Andwith the increase in 17β-E2 concentration,the expression of CIDEc and Plin1 mRNA was in decline. Conclusion 17β-E2 reduces CIDEc and Plin1 mRNA expression in adipocytes,in which inhibited CIDEc and Plin1 expression may contribute to the negative effect of 17β-E2 on lipid droplets.

关 键 词：17Β-雌二醇 脂滴 脂肪细胞 成脂分化 CIDEc Perilipin1 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R622[医药卫生—基础医学]