电针对膜迷路积水模型豚鼠耳蜗形态及水通道蛋白表达的影响  

Effects of electroacupuncture on cochlea morphology and expression of aquaporins in guinea pigs with endolymphatic hydrops

作　　者：蒋丽元[1] 王灿军[1] 倪芳英[1] 陈华德[2] 

机构地区：[1]浙江中医药大学第三临床医学院,杭州310053 [2]浙江中医药大学国际教育学院,杭州310053

出　　处：《中国针灸》2015年第6期579-584,共6页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金资助项目:81373757

摘　　要：目的:探讨电针治疗梅尼埃病的可能调节机制。方法:将40只豚鼠随机分为空白组、模型组、药物组和电针组,每组10只。除空白组外,其余3组采用腹腔注射醛固酮方法造膜迷路积水模型。空白组、模型组采取与电针组相同的固定,不予治疗;药物组予氢氯噻嗪溶液5mg/kg灌胃,每日1次,连续治疗10d;电针组予"百会""听宫"电针治疗,每日1次,连续治疗10d。采用比浊法检测各组豚鼠血清离子浓度,HE染色法观察耳蜗积水程度,免疫组化染色法检测耳蜗水通道蛋白1(AQP1)表达的情况。结果:1空白组未出现膜迷路积水;模型组出现中、重度膜迷路积水;电针组和药物组出现轻度膜迷路积水。2电针组K+、Ca2+浓度高于模型组及药物组(均P<0.01);Na+浓度低于模型组(P<0.01),高于药物组(P<0.01);Cl-浓度高于药物组(P<0.01),但与模型组比较差异无统计意义(P>0.05)。3模型组AQP1表达面积比值较空白组降低(P<0.01);电针组AQP1表达面积比值较模型组升高(P<0.01),较药物组降低,但差异无统计学意义(P>0.05)。结论:电针可减轻豚鼠膜迷路积水,其作用机制可能与上调耳蜗AQP1的表达有关,并受离子浓度变化的影响。Objective To observe the effects of electroacupuncture（EA）on cochlea morphology and expression of aquaporin 1（AQP1）in guinea pigs with endolymphatic hydrops,so as to explore the possible mechanism of EA on endolymphatic hydrops.Methods Forty guinea pigs were randomly divided into a blank group,a model group,a medication group and an EA group,10 guinea pigs in each one.Model of endolymphatic hydrops was established by using intraperitoneal injection of aldosterone.Guinea pigs in the blank group and model group were treated with identical immobilization as EA group but no treatment was given;guinea pigs in the medication group were treated with intragastric administration of hydrochlorothiazide at a dose of 5mg/kg,once a day for consecutive 10 days;guinea pigs in the EA group were treated with EA at＂Baihui＂（GV 20）and＂Tinggong＂（SI 19）,once a day for consecutive 10 days.The serum ionic concentration in each group was tested by turbidimetric method;hematoxylin-eosin staining was used to measure the severity of cochlea hydrops;immunohistochemical method was used to observe the expression of AQP1 in the cochlea.Results① There was no endolymphatic hydrops in the blank group,moderate-severe endolymphatic hydrops in the model group and slight endolymphatic hydrops in the EA group and medication group.② The concentration of K^＋ and Ca^2＋ in the EA group was higher than that in the model group and medication group（all P〈0.01）;the concentration of Na^＋ was lower than that in the model group（P〈0.01）but higher than that in the medication group（P〈0.01）;the concentration of Cl^- was higher than that in the medication group（P〈0.01）,but not significantly different from the model group（P〉0.05）.③ The ratio of expression area of AQP1 in the model group was lower than that in the blank group（P〈0.01）;the ratio of expression area of AQP1 in the EA group was higher than that in the model group（P〈0.01）,and lower than that in the medication group without significa

关 键 词：膜迷路积水 电针 水通道蛋白1 梅尼埃病 

分 类 号：R246.81[医药卫生—针灸推拿学]