固相萃取-超高效液相色谱-串联质谱法同时测定食品接触塑料制品中10种苯并三唑类紫外吸收剂  

Simultaneous determination of ten benzotriazole ultraviolet stabilizers in food contact plastic materials by solid phase extraction and ultra performance liquid chromatography with tandem mass spectrometry

作　　者：勾新磊[1] 赵新颖[1] 池海涛[1] 高峡[1,2] 周明强[1] 刘伟丽[1,2] 

机构地区：[1]北京市理化分析测试中心,有机材料检测技术与质量评价北京市重点实验室,北京100089 [2]北京市食品安全分析测试工程技术研究中心,北京100089

出　　处：《色谱》2015年第6期571-576,共6页Chinese Journal of Chromatography

基　　金：北京市自然科学基金项目(2122018);北京市科学技术研究院萌芽计划项目(2015-02)

摘　　要：建立了固相萃取-超高效液相色谱-串联质谱（SPE-UPLC-MS/MS）同时测定食品接触塑料制品中10种苯并三唑类（BZTs）紫外吸收剂的方法。食品接触塑料制品使用甲醇-二氯甲烷混合溶剂超声提取,C18固相萃取柱净化后用Waters ACQUITY UPLC BEH C18色谱柱（100 mm×2.1 mm,1.7μm）分离,甲醇和0.1%（v/v）甲酸水溶液为流动相进行梯度洗脱,多反应监测（MRM）模式检测,外标法定量分析。结果表明,10种BZTs在线性范围内线性关系良好,线性相关系数（r^2）均大于0.996;检出限为0.6-1.6μg/kg;3个水平下的加标回收率为75.2%-85.3%,相对标准偏差为1.0%-5.7%。该方法准确、简便、快速、检出限低,可用于食品接触塑料制品中10种BZTs的同时检测。A sensitive method was developed for the simultaneous determination of ten benzo-triazole ultraviolet stabilizers in food contact plastic materials by ultra performance liquid chro-matography-tandem mass spectrometry（ UPLC-MS/MS）. The sample was extracted by metha-nol-dichloromethane,and purified by a C18 solid-phase extraction（ SPE）column. The separa-tion was performed by using water containing 0. 1%（ v/v ） formic acid and methanol as the mobile phases with gradient elution at a flow rate of 0. 3 mL/min. The electrospray ionization （ ESI）source in positive ion mode was used for the analysis of the ten benzotriazole ultraviolet stabilizers in multiple reaction monitoring（ MRM）mode. The results showed that the standard curves were obtained with good correlation coefficients（r^2﹥0. 996）in their linear concentration ranges. The limits of detection（ LODs,S/N=3）for the ten benzotriazole ultraviolet stabilizers were in the range of 0. 6-1. 6 μg/kg. The mean recoveries for the ten benzotriazole ultraviolet stabilizers at three spiked levels（ low,medium and high ） were 75. 2%-85. 3% with relative standard deviations of 1. 0%-5. 7%. Ten kinds of food contact plastic materials were tested,and 2,2′-methylenebis（6-（benzotriazol-2-yl）-4-tert-octylphenol）（UV-360）was found in a sample of polyethylene（ PE ） material. The method is accurate,simple,rapid and feasible for the simultaneous determination of benzotriazole ultraviolet stabilizers in food plastic materials.

关 键 词：超高效液相色谱-串联质谱 苯并三唑类化合物 紫外吸收剂 食品接触塑料制品 

分 类 号：O658[理学—分析化学]