玫瑰花中的异黄酮类化合物及其活性研究  

Isoflavones from flower buds of Rosa rugosa and their cytotoxicity

作　　者：李丽梅[1] 娄洁[1] 刘贵有[1] 王欢[1] 杨娟霞[1] 王月德 董伟[1] 周堃[1] 胡秋芬[1] 

机构地区：[1]云南民族大学民族药资源化学国家民委教育部重点实验室,云南昆明650500

出　　处：《中草药》2015年第10期1420-1422,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(21032085;31460144);云南省高校科技创新团队(IRTSTYN 2014-11);云南民族大学研究生创新项目(2014YJY86)

摘　　要：目的研究玫瑰Rosa rugosa花蕾的化学成分。方法运用硅胶、凝胶、MCI-gel树脂及PR-HPLC等多种色谱技术对玫瑰花蕾的化学成分进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从玫瑰花蕾中分离得到了3个异黄酮类化合物,分别鉴定为6,8-二羟基-4',7-二甲氧基异黄酮(1)、樱黄素(2)、红车轴草素(3)。细胞毒活性筛选显示化合物1对A549和PC3细胞的IC_(50)值分别为2.6和3.2μmol/L。结论化合物1为新化合物,命名为玫瑰异黄酮,化合物2和3首次从玫瑰花蕾中分离得到;化合物1表现出较强的细胞毒活性。Objective To study the chemical constituents from the flower buds of Rosa rugosa. Methods The chemical constituents from the flower buds of R. rugosa were isolated by silica gel, MCI-get resin, Sephadex LH-20 column chromatography and high performance liquid chromatography （HPLC） methods. Their structures were elucidated by spectroscopic methods and physicochemical properties. Results Three isoflavones, 6,8-dihydroxy-4＇,7-dimethoxyisoflavone （1）, prunetin （2）, and pratensein （3） were isolated from the flower buds of R. rugosa. Conclusion Compound 1 is a new compound named rosa isoflavone. Compounds 2 and 3 are isolated from R. rugosa for the first time. Compound 1 displays the stronger cytotoxicity against A549 and PC3 cells with IC50 values of 2.6 and 3.2μmol/L, respectively.

关 键 词：玫瑰 异黄酮 细胞毒活性 6 8-二羟基-4’ 7-二甲氧基异黄酮 玫瑰异黄酮 樱黄素 红车轴草素 

分 类 号：R284.1[医药卫生—中药学]