RP-HPLC法同时测定舒胸方中7种有效成分  

Simultaneous determination of seven effective constituents from Shuxiong prescription by RP-HPLC

作　　者：冯彬彬[1] 张建海[1] 阳文武 何世新 

机构地区：[1]重庆三峡医药高等专科学校,重庆404120 [2]重庆万州食品药品检验所,重庆400716

出　　处：《中草药》2015年第10期1477-1480,共4页Chinese Traditional and Herbal Drugs

基　　金：重庆市科委自然科学基金项目(CSTC2012jjA10012)

摘　　要：目的建立RP-HPLC同时测定舒胸方中盐酸川芎嗪、阿魏酸、羟基红花黄色素A、人参皂苷Rg1、人参皂苷Rb1、人参皂苷Re、三七皂苷R1的方法。方法以舒胸方为研究对象，采用RP-HPLC法，以Inertsil ODS-SP（250mm×4．6mm，5μm）为分析柱，乙腈-0．05％磷酸水溶液为流动相，梯度洗脱（0-45min，15％乙腈；45-60min，15％-20％乙腈；60-80min，20％-38％乙腈；80-90min，15％乙腈），检测波长为203nm，柱温30℃，体积流量1．0mL／min，进样量为20μL。结果7种成分盐酸川芎嗪、阿魏酸、羟基红花黄色素A、人参皂苷Rg1、人参皂苷Rb1、人参皂苷Re、三七皂苷R1线性范围分别为0．1010～1．364、0．0640～0．8670、1．020～13．77、1．699～22．95、1．869～25．23、0．1714～2．314、0．5160～6．966μg／mL，线性关系良好，r值均在0．9993～0．9999，平均加样回收率在97．25％～103．52％，RSD均〈3％。结论该方法简便、准确可靠，重复性好，分离度好，适用于同时测定舒胸方中主要7种有效成分。Objective To establish a RP-HPLC method for the determination of ligustrazine hydrochloride, ferulic acid, hydrosafflower yellow A, ginsenoside Rgb ginsenoside Rbb ginsenoside Re, and notoginsenoside R1 in Shuxiong prescription. Methods The Inertsil ODS-SP （250 mm×4.6 mm, 5 μm） was used. The mobile phase, consisting of acetonitrile-0.05% phosphoric acid solution, was programmed for a gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was 203 nm, and the column temperature was 30 ℃. Results The excellent linearity with correlation coefficients （r） of 0.999 3-0.999 9 was obtained. The average recoveries of the seven compounds were 97.25%-103.52% and all RSD values were less than 3%. Conclusion The method appears to be simple, accurate, and well reproducible, which could be used for the simultaneous determination of the above-mentioned seven compounds in Shuxiong prescription.

关 键 词：舒胸方 盐酸川芎嗪 阿魏酸 羟基红花黄色素A 人参皂苷Rg1 人参皂苷RB1 人参皂苷RE 三七皂苷R1 

分 类 号：R286.02[医药卫生—中药学]