低温胁迫对新疆紫草悬浮细胞次生代谢物的影响  

Effects of low temperature stress on secondary metabolites of Arnebia euchroma suspension cells

作　　者：谢腾[1,2,3] 王升[2,3] 张山山[2,3,4] 刘谈[2,3] 郭兰萍[2,3] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]中国中医科学院中药资源中心,北京100700 [3]道地药材国家重点实验室,北京100700 [4]武汉轻工大学,湖北武汉430023

出　　处：《中草药》2015年第10期1525-1532,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81130070;81072989);国家科技支撑项目(2012BAI29B02;2012BAI28B002)

摘　　要：目的通过考察低温胁迫后新疆紫草悬浮细胞次生代谢物量的变化,探讨道地产区特有的环境因子对新疆紫草的作用。方法 4℃低温胁迫新疆紫草悬浮细胞24h后,HPLC法分别测定胁迫前(计为0h)和胁迫后12、24、48、72、168h5个时间点迷迭香酸、紫草酸B、紫草呋喃A、紫草呋喃E、乙酰紫草素、脱氧紫草素、异丁酰紫草素、β,β'-二甲基丙烯酰紫草素、异戊酰紫草素和总紫草素类化合物的量。结果低温胁迫后不同时间点同类化合物的变化趋势基本一致,但其量差异显著,除β,β'-二甲基丙烯酰紫草素外,其他化学成分量的最大值均出现在低温胁迫组。结论低温胁迫可以促进新疆紫草悬浮细胞积累次生代谢物,并对揭示新疆紫草道地性形成的机制有重要作用。Objective By examining the secondary metabolites content changes of the low temperature stressed Arnebia euchroma suspension cells to explore the effects of unique environmental factors in genuine regional areas on A. euchroma in non-genuine regional areas. Methods After stressing A. euchroma suspension cells for 24 h at 4 ℃, rosmarinic acid, lithospermum acid, shikonofuran A, shikonofuran E, acetylshikonin, deoxyshikonin, β,β＇-dimethylacryl shikonin, isovalerylshikonin, and total shikonin compounds were measured by HPLC on six time points, one of which was before the stress and counted as 0 h, and others were in 12, 24, 48, 72, and 168 h after the stress. Results The results showed that the different time points after low temperature stress, similar compounds had the same consistent change trends basically. However, the contents had significant differences. In addition to DAS, the maximum value of all the chemical ingredients were in low stress group. Conclusion Low temperature stress can promote the accumulation of secondary metabolites in A. euchroma suspension cells, and have an important role in revealing the mechanism of the formation of its genuineness.

关 键 词：低温胁迫 新疆紫草 次生代谢物 迷迭香酸 紫草酸B 紫草呋喃A 紫草呋喃E 乙酰紫草素 脱氧紫草素 异丁酰紫草素 β β’-二甲基丙烯酰紫草素 异戊酰紫草素 

分 类 号：R282.21[医药卫生—中药学]