近平滑念珠菌形态多样性及毒力因子的分泌活性研究  

The morphological diversity and virulence factorssecretory activity of Candida parapsilosis

作　　者：李小玲[1] 金方[1] 陈玉如 牟丽丽[1] 刘涛华[1] 吕倩[1] 康颖倩[1] 

机构地区：[1]贵阳医学院微生物学教研室,贵阳550004 [2]贵阳市第五人民医院检验科,贵阳550004

出　　处：《中国真菌学杂志》2015年第2期65-69,共5页Chinese Journal of Mycology

基　　金：国家自然基金(31060006;31260029);贵阳市科技局社会发展与民生计划(筑科合同[2011103]16号);贵州省社会发展科技攻关项目(黔科合SY字[2011]3017号);贵阳市科技局科技创新平台计划(筑科合同2012303号)

摘　　要：目的比较近平滑念珠菌不同临床分离株的菌落和细胞形态及其分泌型天冬氨酸蛋白酶(SAP)酶活性,阐明该病原真菌的形态多样性及毒力差异。方法分别用Lee’s Glc NAc、Lee’s glucose、Spider 3种培养基于37℃培养7株近平滑念珠菌临床分离株,观察各菌株的菌落及细胞形态;用牛血清白蛋白培养基(BSA)于30℃培养7株近平滑念珠菌,通过观察形成晕圈能力的强弱确定其毒力因子(SAP)的分泌活性。结果 7株近平滑念珠菌临床分离株在不同条件下菌落表型有较明显的不同,菌落有光滑型、皱褶型及火山口样型,且染色程度不同,各分离株细胞在形成酵母相或菌丝相的能力方面呈现出不同程度的差异;SAP酶活性观察实验中检测到各菌株水解晕圈大小从2.55 cm到1.55 cm各不相等。结论近平滑念珠菌临床分离株具有形态多样性,各菌株SAP酶活性明显不同,提示菌株间的毒力有明显差异。Objective Compared the colonies and cells morphology and the secreted aspartic proteinase （SAP） enzyme activity of different clinical isolates of Candida parapsilosis,and elucidated the morphological diversity and the differences of virulence of the pathogenic fungi.Method Seven clinical isolates of Candida parapsilosis were cultured respectively on different solid media,and the morphology characteristics of colonies and cells were observed and recorded.Strains were also cultured on solid medium of bovine serum albumin （BSA） at 30 ℃ for determining the secretory activity of the virulence factors （SAP） by observing the proteolytic halos. Result Diverse colonies and phenotypes of the above strains were checked under several sorts of conditions—smooth,crepe and crater in various shades of stained color,and the ability of filamentation were also different from each other among the tested strains.In addition,the sizes of proteolytic halos were various from 2.55 cm to 1.55 cm.Conclusion There are diverse phenotypes which can be shown for the clinical isolates of Candida parapsilosis,and the SAP enzyme activities of them are distinct,which may imply the significant virulent differences among the strains.

关 键 词：近平滑念珠菌 形态多样性 SAP 酶活性 

分 类 号：R379.4[医药卫生—病原生物学]