S100A2转染SGC-7901细胞后对其增殖、凋亡和迁徙能力的影响  

Effect of S100A2 on the ability of proliferation, apoptosis and migration in gastric cancer cells(SGC-7901)

作　　者：张天彪[1] 宿文辉[1] 赵滢[2] 陈鑫莹[2] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学教研室,辽宁沈阳110122 [2]中国医科大学附属盛京医院胃肠营养外科,辽宁沈阳110122

出　　处：《解剖科学进展》2015年第3期299-301,304,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金青年基金(No.81100462)

摘　　要：目的克隆胃粘膜上皮细胞GES-1细胞中S100钙结合蛋白A2 c DNA,构建p SMPUW-NeoS100A2重组表达载体,转染胃癌细胞SGC-7901后,研究S100A2蛋白的表达变化,及其对胃癌细胞增殖、凋亡和迁徙能力的影响。方法应用重组技术构建p SMPUW-Neo-S100A2融合蛋白表达载体,质粒经测序正确后,western-blot检测S100A2蛋白表达水平变化,MTT检测细胞增殖能力、流式细胞仪检测细胞凋亡、划痕实验检测细胞迁徙能力的改变。结果构建的p SMPUW-Neo-S100A2表达质粒,转染SGC-7901细胞后,发现S100A2蛋白表达水平升高,胃癌细胞出现增殖能力下降、凋亡升高和迁徙能力下降的现象。在细胞增殖能力检测中,表达质粒转染细胞后第1天和第2天的细胞增长率分别为18%和32%,而对照组对应为27%和56%,差距有统计学意义(<0.05)。结论S100 A2转染胃癌SGC-7901细胞后使该细胞的增殖能力下降、细胞凋亡程度升高和迁徙能力下降,可以作为一个生物标记物对其进行筛选。Objective We cloned the calcium-binding protein S100A2 c DNA in gastric epithelial cells（GES-1）, and developed p SMPUW-Neo-S100A2 recombinant expression vector. After the transfection of gastric cancer cells（SGC-7901）, we studied the change of S100A2 expression, and its impact on proliferation, apoptosis and migration ability of gastric cancer cells. Methods Employing recombination technique, p SMPUW-Neo-S100A2 fusion protein expression vector was developed. After sequencing the plasmid, the change of S100A2 protein expression was carried out by Western-blot. Then, cell＇s proliferation, apoptosis and migration were characterized using MTT, flow cytometry and scratch test respectively. Results The p SMPUW-Neo- S100A2 expressed plasmids were observed to show increased S100A2 expression level after transfection in SGC-7901 cells. And gastric cancer cells showed decreased proliferation activity, increased apoptosis ratio and reduced migration ability. The growth rates were 18% and 32% at the first day and the second day after transfection in SGC-7901 cells, obviously lower than control group（P〈0.05, 27% and 56% respectively）. Conclusion The transfection of S100A2 into SGC-7901 inhibits the cell proliferation and migration ability, S100A2 transfection can be used as one biomarker.

关 键 词：S100钙结合蛋白A2 胃粘膜上皮细胞GES-1 细胞增殖 细胞凋亡 细胞迁徙 

分 类 号：Q813.1[生物学—生物工程]