始旋链霉菌HCCB10218产生的诺卡胺类物质的发现  

The discovery of nocardamines from Streptomyces pristinaespiralis HCCB10218

作　　者：李亚南[1,2] 饶敏[2] 魏维[2] 陈代杰[1] 戈梅[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海来益生物药物研究开发中心有限责任公司,上海200240

出　　处：《中国抗生素杂志》2015年第6期414-418,共5页Chinese Journal of Antibiotics

基　　金：国家科技部卫生部重大新药创制专项(No.2012ZX09301002-003)

摘　　要：目的鉴定从始旋链霉菌中发现的诺卡胺类物质。方法利用超高效液相色谱-质谱联用(UPLC-MS)技术,结合二级质谱解析、数据库检索等多种手段分析和鉴定始旋链霉菌HCCB10218菌株发酵产生的诺卡胺类产物,并通过生物信息学分析解析其生物合成途径。结果在始旋链霉菌HCCB10218的发酵提取物中发现了4个诺卡胺类物质(A^D),其中化合物B为新化合物。结论诺卡胺类物质是首次在始旋链霉菌中发现。该结果丰富了放线菌活性代谢产物的多样性,同时补充了诺卡胺类物质的生物合成途径。Objective To identify nocardmine and its derivatives from S. pristinaespiralis. Methods Nocardmine and its derivatives from metabolites of S. pristinaespiralis HCCB 10218 were analyzed and identified using UPLC-MS coupled with two-stage mass spectrometry approach and database search and the pathways of the biosynthesis of nocardmine and its derivatives were deduced through bioinformation analysis. Results Four kinds of nocardmine and its derivatives（A-D）, including compound B, a brand-new one, were identified from the fermentation broth of S. pristinaespiralis HCCB10218. Conclusion Nocardmine and its derivatives were first discovered from S. pristinaespiralis, which enriched active metabolites of Actinomyces and provided the new pathways for the biosynthesis of nocardmine and its derivatives.

关 键 词：始旋链霉菌 超高效液相色谱-质谱联用技术 诺卡胺 

分 类 号：Q93[生物学—微生物学]