妇科门诊病人HPV分型检测及感染情况分析  

Analysis of Human Papillomavirus Genotype and Infection in Out-patients of Gynecology Department

作　　者：李灿 陈伟 赵静 兰长贵 

机构地区：[1]核工业416医院皮肤科,成都610051

出　　处：《西南军医》2015年第3期253-255,共3页Journal of Military Surgeon in Southwest China

摘　　要：目的分析人乳头瘤病毒（HPV）感染亚型及分布特点。方法采用聚合酶链反应（PCR）体外扩增和反向斑点杂交-基因芯片技术,对1179例成都市成华区妇科门诊妇女的宫颈脱落细胞标本进行检测,对检测结果进行统计学分析。结果 1179例临床标本中共检出HPV感染224例,感染率为19.0%。21种基因亚型检出20种,未检出HPV43低危亚型。感染率最高的亚型是高危16型（占22.3%）,其次是52型（占21.9%）和58型（占15.2%）。单一亚型感染率为13.7%,多重感染为5.3%。25岁以下年龄组、25-35岁、36-45岁、46-55岁、55岁以上年龄组HPV检出率分别为19.6%、20.2%、15.2%、21.0%及34.0%。结论成都市成华区受检女性HPV感染以单一感染和高危感染为主,感染率最高的亚型是高危16型。不同年龄分层的感染率略有不同。Objective To investigate the human papillomavirus （HPV） infection subtype and its distribution. Methods Polymerase chain reaction （PCR） and reverse dot blot hybridization and DNA microarray technology were applied in detecting the 1179 cervical cytology specimens from the out-patients of Gynecology Department, Chenghua District, Chengdu; the results were statistically analyzed. Results Out of the 1179 specimens, HPV infection was found in 224 specimens, the infection rate was 19.0%; out of the 21 known sub- types, except the low-risk type HPV-43, 20 subtypes were detected; the 3 subtypes with the top infection rate was high-risk genotype HPV-16（22.3%）, HPV-52 （21.9%） and HPV-58 （15.2）; the single HPV subtype infection rate was13.7% while the multiple HPV subtype infection rate was 5.3%; the detection rate of HPV infection in each age group （〈25, 25 to 35, 36 to 45, 46 to 55 and 〉55） was 19.6%, 20.2%, 15.2%, 21.0% and 34.0% respectively. Conclusions Single infection and high-risk infection took the main part in the HPV infection in the enrolled females of Chenghua District, Chengdu; there existed a slight difference in infection rates of different age groups.

关 键 词：人乳头瘤病毒 基因亚型 导流杂交 宫颈癌 

分 类 号：R446.1[医药卫生—诊断学]