Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99–125 lipase via adsorption  

作　　者：Ya Li Jicheng Hu Pingfang Han 

机构地区：[1]College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University [2]Department of Environment and Resource, Nantong Science and Technology College

出　　处：《Chinese Journal of Chemical Engineering》2015年第5期822-826,共5页中国化学工程学报（英文版）

基　　金：the National Basic Research Program of China(2009CB724700);the Foundation of Jiangsu Province of China for College Postgraduate Students in Innovation Engineering(CXZZ12_0440)

摘　　要：Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffrac- tion, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99-125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of 41.5 mg· g^-1 support and enzyme activity of 2631.6 U· （g support）^-1. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusabilitV compared with free lipase.Magnetically modified palygorskite composites were synthesized with γ-Fe2O3 dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99–125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of41.5 mg·g-1support and enzyme activity of 2631.6 U·(g support)-1. The immobilized lipase exhibit better thermal and broader p H stability and excellent reusability compared with free lipase.

关 键 词：Magnetically modified palygorskite Immobilized lipase Adsorption Stability Reusability 

分 类 号：TB332[一般工业技术—材料科学与工程] O629.8[理学—有机化学]