顺铂对宫颈癌裸鼠移植瘤模型淋巴转移的影响及作用机制  

The effect of cisplatin on the lymphatic metastasis of cervical carcinoma xenografts model of nude mice and its mechanism

作　　者：陈云飞[1] 王朝霞[1] 李莉[2] 

机构地区：[1]山西医科大学,山西太原030001 [2]山西医科大学第一医院妇产科,山西太原030001

出　　处：《中国计划生育和妇产科》2015年第6期6-8,F0003,共4页Chinese Journal of Family Planning & Gynecotokology

基　　金：山西省自然科学基金(项目编号:2007011121)

摘　　要：目的探讨顺铂(DDP)对宫颈癌淋巴转移作用的影响及其作用机制。方法将14只裸鼠建立人宫颈癌裸鼠移植瘤模型后,随机分为对照组和顺铂组各7只,对照组裸鼠腹腔注射PBS缓冲液,顺铂组裸鼠腹腔注射顺铂。观察裸鼠淋巴转移情况,采用病理学方法认证淋巴结及淋巴结内部的宫颈癌浸润,采用免疫组化方法检测肾小球足突细胞膜黏蛋白(Podoplanin)和胸苷磷酸化酶(thymidine phosphorylase,TP)的表达。结果顺铂组对淋巴转移有明显抑制作用,顺铂组淋巴转移个数及淋巴转移率均低于对照组(P<0.05)。顺铂组Podoplanin和TP的表达低于对照组(P<0.05)。结论应用顺铂治疗后,对裸鼠淋巴转移有明显的抑制作用。淋巴结中Podoplanin和TP的表达下调,抑制宫颈癌移植瘤的增殖和侵袭能力,可能是顺铂抑制宫颈癌淋巴转移的机制之一。Objective To detect the effect of cisplatin （ DDP ） on the lymphatic metastasis of cervical carcinoma and its mechanism.Methods The human cervical carcinoma xenografts model was established in 14 nude mice, which were randomly divided into control group were and DDP group with 7 cases in each group.Mice in control group were injected PBS, mice in DDP group were injected cisplatin.The lymphatic metastasis was observed, invasion of cervical cancer in lymph nodes and the internal were certified by pathological methods, the expression of Podoplanin and thymidine phosphorylase （ TP ） were detected by immunohistochemical method.Results Cisplatin had an inhibitory effect for lymphatic metastasis.The number and incidence of lymphatic metastasis of DDP group were lower than control group（P〈0.05）.The expression of Podoplanin and TP in DDP group were lower than the control group （ P 〈0.05 ） .Conclusion The lymphatic metastasis in nude mice were inhibited after treatment of cisplatin, the expression of Podoplanin and TP was down-regulated.The proliferation and invasion of xenografts was inhibited may be one of the mechanisms of cisplatin inhibited lymphatic metastasis of cervical carcinoma.

关 键 词：宫颈肿瘤 顺铂 淋巴转移 PODOPLANIN 胸苷磷酸化酶 

分 类 号：R332[医药卫生—人体生理学]