PGC-1α参与调节向心运动中骨骼肌白介素6的抗炎效应  

The Effects of Concentric Exercise on the Anti-inflammation of Interleukin-6 in Skeletal Muscle

作　　者：宋超[1] 张竞文[2] 薄海[2] 吉力立[2,3] 张勇[2] 

机构地区：[1]北京体育大学研究生院,北京100084 [2]天津体育学院天津市运动生理学与运动医学重点实验室 [3]美国明尼苏达大学运动科学学院

出　　处：《中国运动医学杂志》2015年第4期329-333,共5页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金重大国际合作项目(31110103919);天津市应用基础与前沿技术研究计划重点项目(14JCZDJC36600);国家科技支撑计划课题(2012BAD44B01-02);天津市科技创新体系创新平台计划专项(10SYSYJC28400)

摘　　要：目的:探讨一次性向心运动对白介素6(IL-6)抗炎效应的影响,及PGC-1α在其中的角色。方法:2月龄雄性C57BL/6小鼠70只,建立一次性上坡跑运动模型(速度10 m/min,坡度5°),分别选取运动前(Control),运动30 min和60 min(E30,E60),运动恢复期30 min、60 min、3 h和6 h(PE30、PE60、PE3h、PE6h)为实验观测点,每组10只动物。二氯荧光素法检测骨骼肌线粒体活性氧(ROS)生成速率,硫代巴比妥酸法检测骨骼肌MDA含量,荧光定量PCR法检测骨骼肌白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)、过氧化物酶体增殖活化受体γ共激活因子-1α(PGC-1α)和核因子-κB(NF-κB)p65 m RNA表达。结果:与Control组相较,ROS生成速率在E30、E60、PE30、PE60和PE3h组显著升高(P<0.01);MDA含量在各组均无显著性变化(P>0.05);IL-6 m RNA表达水平在E30、E60和PE30组显著升高(P<0.05);TNF-αm RNA表达水平在PE60和PE3h组显著升高(P<0.01);PGC-1αm RNA表达水平在E30、E60、PE30、PE60和PE3h组显著升高(P<0.01);NF-κB p65 m RNA表达水平在PE60和PE3h组显著升高(P<0.01)。结论:一次性向心运动可诱导具有抗炎效应的IL-6脉冲式表达升高。PGC-1α可能通过抑制ROS的氧化应激损伤及NF-κB表达两条途径抑制炎症,参与调节向心运动中骨骼肌IL-6的抗炎效应。Objective To investigate the impact of an acute concentric exercise on the anti-inflammation of IL-6 and the role of PGC-1α in such process. Methods 70 two-month old male C57BL/6 mice were divided into control group,30-min exercise group（E30）,60-min exercise group（E60）,and 30-min,60-min,3-hour,and 6-hour after exercise groups（PE30,PE60,PE3 h and PE6 h,respectively）. Mice in exercise groups performed a continuous concentric exercise at the speed of 10m/min with 5% slope. Mitochondrial ROS generation rate,contents of MDA,IL-6,TNF-α,PGC-1α,and NFκB-p65 m RNA were determined. Results As compared to the control group,（1）mitochondrial ROS generation rate increased significantly in E30,E60,PE30,PE60 and PE3 h groups（P〈0.01） and the MDA content in all groups remained unchanged（P〈0.05）;（2）IL-6 m RNA level in E30,E60 and PE30 groups,TNF-α m RNA level in PE60 and PE3 h groups,PGC-1αm RNA content in E30,E60,PE30,PE60 and PE3 h groups,and NF-κB p65 m RNA level in PE60 and PE3 h groups increased significantly（P〉0.05 ~P〈0.01）. Conclusion An acute concentric exercise could induce higher impulse-type expression of muscle-derived interleukin-6 mediated by PGC-1α through inhibition of oxidative damage induced by ROS and inactivation of NF-κB.

关 键 词：向心运动 骨骼肌 IL-6 PGC-1Α 抗炎效应 

分 类 号：R87[医药卫生—运动医学]