度他雄胺和“扶正抑瘤”中药复方对人前列腺癌PC-3细胞转移抑制效应的对照研究  

Effect Contrast Research of Dutasteride and “Qi Strengthening and Tumor Inhibiting”Chinese Herbal Compound Controlling Anoikis of Human Prostate Cancer PC-3 Cells

作　　者：周仕轶[1] 王林[2] 邵继春 张蜀武[1] 

机构地区：[1]成都中医药大学临床医学院/附属医院,四川成都610075 [2]邛崃市医疗中心医院,四川邛崃611500 [3]核工业416医院,四川成都610051

出　　处：《成都中医药大学学报》2015年第2期10-15,共6页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省卫生厅科研课题(编号:120466);四川省教育厅科研项目(编号:12ZB210)

摘　　要：目的:通过失巢凋亡抑制,对照研究新型5α还原酶抑制剂—度他雄胺和由人参、姜黄、肿节风组成的"扶正抑瘤"中药复方调控人前列腺癌PC-3细胞转移的效应。方法:采用人前列腺癌PC-3细胞体外培养的方法;MTT法检测PC-3细胞对度他雄胺和中药复方含药血清的敏感度,并确定两者的浓度梯度;软琼脂集落形成实验法观察两者对PC-3细胞失巢条件下增殖的影响;以Poly-HEMA包被培养细胞悬浮生长和琼脂糖电泳实验观察两者对PC-3细胞失巢凋亡的诱导作用;并采用流式细胞仪PI单染和AV-PI双染定量检测各组细胞凋亡率。结果:MTT比色法确定度他雄胺的浓度梯度为15%(低剂量),30%(中剂量)和45%(高剂量),中药复方为30%(中剂量);软琼脂集落实验表明度他雄胺45%含药血清组和中药复方含药血清组对细胞集落形成数量具有抑制作用(P<0.05),2组间比较无差异(P>0.05);琼脂糖电泳显示度他雄胺45%含药血清组和中药复方含药血清组出现较明显凋亡DNA-Ladder条带;流式细胞检测PI单染显示,度他雄胺45%含药血清组和中药复方含药血清组可提高PC-3细胞失巢凋亡率(P<0.01),AV-PI双染显示度他雄胺各浓度组和中药复方含药血清组仅具有提高细胞早期失巢凋亡率的趋势(P>0.05)。结论:度他雄胺可抑制人前列腺癌PC-3细胞在失巢条件下增殖,并呈现一定的浓度依赖性,中药复方也可抑制人前列腺癌PC-3细胞在失巢条件下增殖,两者效应相当,两者均对PC-3细胞失巢凋亡具有相似诱导作用,但对细胞早期凋亡诱导作用均有限。Objective： To study the controlling effect both the new type 5α reductase （dutasteride） and Chinese herbal compound （ginseng, turmeric and sarcandra glabra） having on human prostate cancer PC-3 cells by cell anoikis inhibition. Methods： PC-3 cells were cultivated in vitro. MTY was used to measure the PC-3 cells＇ sensitivities to dutasteride serum and herbal compound serum to determine serum concentration gradient. The effects of both dutasteride and herbal compound on the multiplication of PC-3 cells were observed by colony formation test in soft agar. The apoptotic effects of the two on PC-3 cells were studied by Poly-HEMA coated cells cultivation and agar electrophoresis. Cell apoptotic rates were measured by both PI and AV-PI methods in flow cytometer. Results： Dutasteride serum concentration gradient was determined as 45% serum （high dose） group, 30% serum （medium dose） group and 15% serum （low dose） group with herbal compound as 30% serum （medium dose） group by MTr. The 45% serum group of dutast- eride and herbal compound group could inhibit the number of cell colony formation compared with control group （ P 〈 0. 05 ） with no difference between the two groups （P 〉 0. 05 ） in soft agar. The 45% serum group of dutasteride and herbal compound group made PC- 3 cells appear obvious apoptotic DNA ladder bands through agar electrophoresis. Dutasteride 45% serum group and herbal compound group could enhance PC-3 cells＇ anoikis rates （P 〈 0.05 ） by PI test but only had the tendency to enhance PC-3 cells＇ early anoikis rates （P 〉 0. 05） by AV-PI test in flow cytometer. Conclusion ： Dutasteride can inhibit PC-3 cells multiplication under anchorage independent condition with dose dependent effect. The Chinese herbal compound has similar effect to inhibit PC-3 cells multiplication as dutasteride. Both of them can also induce PC-3 cells to anoikis but have limited ability to induce them to early anoikis.

关 键 词：度他雄胺 中药复方 前列腺癌 PC-3细胞 失巢凋亡 

分 类 号：R289[医药卫生—方剂学] R273[医药卫生—中药学]