间接ELISA与中和试验检测O型FMDV疫苗免疫猪血清抗体的比较研究  

Comparative study on indirect ELISA and neutralization test of O type FMDV vaccine of porcine serum antibody

作　　者：刘清源[1] 向华 黄元 陈晶 向蓉 郑海学[3] 刘宇[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省农科院动物卫生研究所/广东省兽医公共卫生公共实验室,广东广州510640 [3]中国农业科学院兰州兽医研究所/家畜疫病病原微生物国家重点实验室,甘肃兰州730046

出　　处：《广东农业科学》2015年第9期110-114,共5页Guangdong Agricultural Sciences

基　　金：广东省科技厅省重点实验室建设支撑项目(2012A061100006);广东省农业科学院院长基金(201530);广东省主体机构创新能力建设专项(2011);科技部农业科技成果转化资金(2013GBZE000352)

摘　　要：血清中和抗体滴度的高低,体现了血清对病毒感染的中和能力,是评价疫苗免疫效果的重要指标。对国内3个大型猪场共120头猪的血清样品分别进行O型口蹄疫病毒ELISA和病毒中和试验,并分别比较两种方法的符合率和阳性检出率。结果表明,两种方法的阳性符合率和阴性符合率分别为77.3%和72.2%,总符合率为75%,均能有效检测猪血清中的口蹄疫抗体水平,但是采用间接ELISA检测血清抗体,存在一定比例的假阳性或假阴性。病毒中和试验更适合进行口蹄疫血清学的检测。The serum neutralizing antibody titer reflects the ability of serum neutralization on virus infection. It is an important index for vaccine evaluation. In this study,the O type FMDV of 120 serum samples collected from three domestic large-scale pig farms were detected by ELISA and VNT, and the coincidence rates and positive detection rate of the two methods were compared. The results showed that with the two methods,the positive coincidence rates and negative coincidence rates were 77.3% and 72.2%,the total coincidence rate was 75%. Both of the methods could effectively detect FMD antibody level in serum of pigs. However,there was a certain proportion of false positive or false negative results by indirect ELISA. NT was more suitable for FMD serological monitoring and survey.

关 键 词：口蹄疫 病毒中和试验 酶联免疫吸附试验 符合率 

分 类 号：S852.52[农业科学—基础兽医学]