家蝇抗菌肽的提取及其对凡纳滨对虾抗病力的影响  

Extraction of antibacterial peptides from Musca domestica and their effects on disease resistance of Litopenaeus vannamei

作　　者：李林[1,2] 孙育平 黄燕华 陈冰 曹俊明 王国霞 李永娟 马永萍[1,2] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]广东省农科院动物科学研究所,广东广州510640

出　　处：《广东农业科学》2015年第9期120-125,共6页Guangdong Agricultural Sciences

基　　金：广东省自然科学基金(S2012010008837)

摘　　要：采用超声波诱导的方式筛选出生长状况良好的蝇蛆,通过组织匀浆、乙酸铵浸提、热处理、冻融处理、盐析、透析、蒸馏浓缩等方法提取家蝇抗菌肽,应用分光光度法测定其浓度和提取率,比浊法测定其抗菌活性。在基础饲料中分别添加0、40、80、120 mg/kg家蝇抗菌肽制成4组试验饲料,饲养凡纳滨对虾6周后采用注射法进行白斑综合征病毒攻毒试验,攻毒期为52 h。结果显示,4日龄家蝇繁育的蝇蛆经刺激后生长状况良好,产量最高;提取的家蝇抗菌肽浓度为25.05 mg/m L,提取率为2.36%;提取液稀释11倍后,其对铜绿假单胞菌的抑菌率为21.05%,抗菌活力为24.58 U。与未添加组相比,40、80 mg/kg组累积死亡率显著降低,各添加组的相对保护率分别为42.42%、33.33%和9.09%。结果表明,该提取工艺可保证抗菌肽的提取率,保持其稳定性和抗菌活性;在饲料中添加该抗菌肽,可提高凡纳滨对虾抗白斑综合征病毒的能力。This experiment determined the extraction process of antibacterial peptides from Musca domestica and evaluated the effects of antibacterial peptides of M. domestica on anti-white spot syndrome virus capacity in Litopenaeus vannamei. Maggots with good growth were screened out by ultrasonic induced method,and then antibacterial peptides were extracted through tissue homogenate,ammonium acetate extraction,heat treatment,freezethaw treatment,salting out,dialysis,distillation and concentration,the concentration and yield were determined by spectrophotometry,the antibacterial activity was determined by turbidimetry,finally,shrimps were fed with four experimental diets that prepared by supplementing into the basal diet with 0 mg/kg,40 mg/kg,80 mg/kg and 120 mg/kg antibacterial peptides of M. domestica. After 8-week feeding trial,shrimps were injected with WSSV and fed for 52 hours. The results showed that,at the age of 4 days,the maggots bred by M. domestica were in good growth conditions after stimulation,and the yield was the highest. The concentration of antimicrobial peptides was 25.05 mg/m L,the peptide extracting ratio was 2.36%. Diluted by 11 times,the inhibitory rate and antibacterial activity against Pseudomonas aeruginosa were 21.05% and 24.58 U. Compared with non-supplemented group,the accumulative mortality rates of 40 mg/kg and 80 mg/kg groups were significantly lower. The relative percent survival rates of supplemented groups were 42.42%,33.33% and 9.09%. It indicated that the extraction process could improve the extraction efficiency and ensure the stability and antibacterial activity of antibacterial peptides. The supplementation of antibacterial peptides into diets could improve the anti-white spot syndrome virus capacity in L. vannamei.

关 键 词：家蝇抗菌肽 提取率 抗菌活性 抗病力 

分 类 号：S945.1[农业科学—水产养殖] Q966[农业科学—水产科学]