苦参碱对四氯化碳肝损伤大鼠炎性因子的影响  

Effects of Matrine on inflammatory factor in rats with liver injury of carbon tetrachloride

作　　者：赵岩[1] 

机构地区：[1]南阳医学高等专科学校,河南南阳473000

出　　处：《中医药临床杂志》2015年第5期727-728,共2页Clinical Journal of Traditional Chinese Medicine

摘　　要：目的:观察苦参碱对对四氯化碳(CCl4)诱导急性肝损伤大鼠炎症相关因子的影响。方法:将健康雄性SD大鼠正常喂养l周后随机分为正常对照组,损伤模型组,苦参碱低、高剂量组(10,50 mg·kg-1),连续给药7d,末次灌胃给药1h后,腹腔注射CCl4原液建立急性肝损伤大鼠模型,16h后以比色法测定丙氨酸氨基转移(ALT),门冬氨酸氨基转移酶(AST)的含量;采用ELISA法分别检测各组大鼠血清肿瘤坏死因子-d(TNF-a),白细胞介素6(IL-6)的含量。结果:苦参碱能降低四氯化碳肝损伤大鼠ALT和AST水平,降低大鼠血清肿瘤坏死因子-a(TNF-a),白细胞介素6(IL-6)的含量。结论:苦参碱对四氯化碳造成的急性肝损伤具有保护作用,机理与降低炎性因子的作用有关,此作用有助于化学物质引起的急性肝损伤治疗。Objective： To observe the effects of Matrine on inflammatory factor in rats with acute liver injury of carbon tetrachloride. Methods： healthy male SD rats were randomly divided into normal control group,model group,low-dose and high-dose matrine group（10,50 mg·kg-1）after normal feeding for a week. The experimental rats were administrated with Matrine by ig for 7 days,one hours after the last administration,the experimental rats were injected carbon tetrachloride to copy the acute liver injury model except control group. The contents of tumor necrosis factor-a（TNF-a）,interleukin-6（IL-6）,alanine aminotransferase（ALT）and aspartate aminotransferase（AST）were investigated by elisa and colorimetric methods. Results： Matrine can reduce ALT,AST,TNF-a and IL-6 levels in rats with liver injury induced by carbon tetrachloride. Conclusion： Matrine has the protective effect on acute liver damage induced by CCl4,the mechanism is related to the reduction of inflammatory factor. This effect is beneficial to the treatment of acute liver injury caused by chemical substances.

关 键 词：苦参碱 四氯化碳 肝损伤 炎性因子 

分 类 号：R243[医药卫生—中医临床基础]