百合各株系中秋水仙碱提取方法优化及含量测定  

Determination of colchicine and Optimization of extraction method in Lilium brownii

作　　者：姚红锐[1] 尚靖[2] 柳军[2] 卜长秀 

机构地区：[1]安徽中医药大学第一附属医院药学部,安徽合肥230031 [2]中国药科大学新药筛选中心,南京210009 [3]武警安徽省总队医院,安徽合肥230061

出　　处：《中医药临床杂志》2015年第5期729-731,共3页Clinical Journal of Traditional Chinese Medicine

摘　　要：目的:对百合各株系中秋水仙碱进行提取方法优化并建立含量测定方法。方法:采用正交实验设计和HPLC法进行提取工艺优化和含量测定。结果:百合各株系中秋水仙碱含量普遍高于对照组,对提取条件进行探讨,确定了最佳工艺条件)。结论:优化及测定方法准确可靠,可用于选育优良品种的质量控制。Objective：To establish a method for the determination of colchicine and optimize the extraction methods in Lilium brownii. Methods： Extraction process was optimized and determined through Orthogonal experimental design and HPLC method. Results ： The Contents of colchicine was higher than the control group .The extraction conditions were dis- cussed, and the optimum process conditions were determined. Conclusion： The method was precise and can be used for quality control of fine variety bredding.

关 键 词：百合 秋水仙碱 提取方法 含量测定 

分 类 号：R284.2[医药卫生—中药学]