基于银纳米三角片与罗丹明6G的荧光共振能量转移法检测钴离子  

Fluorescence Resonance Energy Transfer Detection of Cobalt Ions by Silver Triangular Nanoplates and Rhodamine 6G

作　　者：张秀清[1] 彭君[1] 凌剑[1] 刘朝娟[1] 曹秋娥[1] 丁中涛[1] 

机构地区：[1]云南大学化学科学与工程学院,教育部自然资源药物化学重点实验室,云南昆明650091

出　　处：《光谱学与光谱分析》2015年第4期951-955,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21105087);云南省应用基础研究计划项目(2011FB014)资助

摘　　要：以罗丹明6G和牛血清白蛋白的复合物为荧光探针,银纳米三角片为猝灭剂,研究了银纳米三角片与荧光复合物的荧光共振能量转移现象,建立了测定钴离子的荧光分析法。研究发现,一定浓度的荧光复合物与银纳米三角片混合后,由于荧光复合物在银纳米三角片上吸附而发生荧光共振能量转移,荧光猝灭达到80%左右。当钴离子存在时,银纳米三角片与罗丹明6G荧光共振能量转移被破坏,荧光逐渐恢复。随着钴离子浓度的增加,体系荧光值的恢复率（I/I0）与钴离子的浓度（cCo^2＋）有良好的线性关系,线性回归方程为I/I0=1.054＋21.72 cCo^2＋,相关系数r^2为0.996 2。通过对自然水样进行加标回收检测,实现了钴离子的定量检测,回收率在90.4%~115.1%之间。建立了一种可靠的选择性检测钴离子的荧光分析方法。In the present paper ,the authors studied fluorescence resonance energy transfer （FRET ） phenomenon between silver triangular nanoplates and bovine serum albumin （BSA ）/Rhodamine 6G fluorescence complex ,and established a fluorescence method for the detection of cobalt ions .We found that when increasing the silver triangular nanoplates added to certain concen‐trations of fluorescent bovine serum albumin （BSA ） /Rhodamine 6G complex , the fluorescence of Rhodamine 6G would be quenched up to 80% due to the FRET between the quencher and donor .However ,in the presence of cobalt ions ,the disassoci‐ation of the fluorescent complex from silver triangular nanoplates occurred and the fluorescence of the Rhodamine 6G recovered . The recovery of fluorescence intensity rate （I/I0 ） has a good relationship with the cobalt ion concentration （cCo^2＋ ） added . Thus ,the authors developed a fluorescence method for the detection of cobalt ions based on the FRET of silver triangular nano‐plates and Rhodamine 6G .

关 键 词：罗丹明6G 牛血清白蛋白 银纳米三角片 荧光共振能量转移 钴离子 

分 类 号：O657.3[理学—分析化学]