猪血清免疫球蛋白IgG的分离纯化及抗大肠杆菌作用  

Separation and purification of IgG from pig serum and the determination of concentration

作　　者：孟腾飞[1] 韩愈杰[1] 王敬[1] 魏萌[1] 吴国江[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071000

出　　处：《饲料工业》2015年第7期34-37,共4页Feed Industry

摘　　要：试验旨在从猪血清中分离纯化得到纯度较高的免疫球蛋白Ig G。试验采用硫酸铵分步沉淀法从猪血清中粗提猪血清免疫球蛋白,经DEAE52凝胶柱进行纯化,将纯化得到的免疫球蛋白进行SDS-PAGE电泳检测,并通过紫外分光光度计法测定其含量。试验结果显示,粗提可以得到免疫球蛋白Ig G,纯化后的lg G经SDS-PAGE电泳检测,检测出两条带,分别为Ig G的重链和轻链,分子量分别为56.72 k D和26.58 k D,通过紫外分光光度计法测得的Ig G浓度为2.577 mg/ml。结果提示,从猪血清中能分离纯化到免疫球蛋白Ig G,为实际工艺化生产提供理论依据。The purpose of the present study wasto separate and purify Ig G from pig serum. Weextracted pig serum immunoglobulin（Ig G） by us-ing the ammonium sulfate by- step precipitationmethod. After being filtered by DEAE52 gel column,the purified Ig G was detected its purity by SDS-PAGE electrophoresis and had its quantity de-termined by ultraviolet spectrophotometer. The results showed that Ig G was able to be obtained afterbeing roughly extracted from pig serum. SDS-PAGE electrophoresis of the purified Ig G detected twobands： one is the heavy chain and the other is light chain,the molecular weight of which was 65 k Dand 25 k D, respectively. The concentration of Ig G was 2.577 mg/ml by using ultraviolet spectrophotom-eter determination method. These results indicated that we separate and purify Ig G from pig serum.The results provide the experimental basis of efficiency degradation straw.

关 键 词：免疫球蛋白 硫酸铵沉淀法 DEAE52凝胶柱 SDS-PAGE电泳 

分 类 号：S816.75[农业科学—饲料科学]