玫烟色棒束孢几丁质诱导蛋白的培养条件研究  

Study on the Cultural Condition of Protein Induce by Chitin from Isaria fumosorosea Mycelium

作　　者：邹时幸 SHAUKAT Ali 罗莹 郝永芬 任顺祥[1] 黄振[1] 

机构地区：[1]教育部生物防治工程研究中心/华南农业大学农学院,广州510642

出　　处：《中国生物防治学报》2015年第2期273-279,共7页Chinese Journal of Biological Control

基　　金：国家"863"计划项目(2011AA10A204-5);广东省高等学校科技创新重点项目(CXZD1135);广东市科技计划项目(201300000107)

摘　　要：玫烟色棒束孢是重要的昆虫病原真菌之一。本文在测定菌丝总蛋白及SDS-PAGE检测的基础上,比较了不同时间和温度下玫烟色棒束孢Isaria fumosorosea经几丁质诱导前后所产生的蛋白差异。结果表明,PD(potato dextrose)和BM(basic medium)培养液中不含几丁质的对照组(contrast,CK)和加入几丁质诱导的处理组(treatment,T)中菌丝总蛋白含量都随培养时间的延长而增加,且对照组与处理组间菌丝蛋白总浓度差异显著。第5 d在BM培养液中对照组与处理组菌丝总蛋白浓度差异最显著。在不同温度条件下,PD和BM培养液中的菌丝蛋白总浓度在25℃时最高、在31℃时浓度最低,对照组与处理组间蛋白总浓度差异显著;从SDS-PAGE上可以看出,在第5 d、21℃时对照组与处理组间的蛋白条带差异最显著。结合以上信息,玫烟色棒束孢几丁质诱导蛋白的最佳培养条件为BM培养液、在25℃条件下培养5 d。Isaria fumosorosea is one of the most important entomopathogenic fungi. Protein diversity in Isaria fumosoroseus induced by chitin at different culture conditions or not was determined by SDS-PAGE. Protein content of both the chitin-induced mycelium and the CK increased with the increase in incubation time in the potato dextrose agar （PDA） medium and the basic medium （BM）, while there were obvious differences in protein content between the CK and the chitin-induced treatment at different culture time. The difference of protein content between the CK and the treatment was significant, in BM, the difference of protein content was highly significant on the 5th day. Under different temperatures, the CK and the treatment both showed the highest protein concentration at 25 ℃ and the lowest protein concentration at 31 ℃ for both PDA and BM. Protein concentration differed significantly between the CK and the treatment. SDS-PAGE showed the greatest difference in protein bands between the CK and the treatment on the 5th d and under 21 ℃ in BM. The results indicate that chitin induction at 21 ℃ for 5 days in BM is optimal.

关 键 词：玫烟色棒束孢 几丁质诱导蛋白 培养条件 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]