高效液相色谱法测定牛组织中三氯苯唑及其代谢物残留量的研究  

Study on Detection Method of Triclabendazole and Metabolite Residues in Cattle Tissues by HPLC

作　　者：李丹[1] 李莉 张玉洁[1] 王鹤佳[1] 李倩[1] 薛毅[3] 仲锋[1] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]宁夏回族自治区兽药饲料监察室,银川750002 [3]中国动物疫病预防控制中心,北京100026

出　　处：《中国兽药杂志》2015年第6期53-57,共5页Chinese Journal of Veterinary Drug

摘　　要：建立了一种可同时检测牛组织中三氯苯唑及其代谢物三氯苯唑酮药物残留的高效液相色谱-紫外检测法。牛组织样品经乙腈提取,异辛烷去脂肪,PPL柱净化。以0.02 mol/L乙酸铵溶液和乙腈溶液为流动相,流速为1.0 m L/min,检测波长296 nm。结果表明,三氯苯唑、三氯苯唑酮在20~1200 ng/m L范围内,峰面积与浓度呈良好的线性关系,R2均大于0.999。方法的最低定量限为50μg/kg。三氯苯唑、三氯苯唑酮在50~600μg/kg的浓度添加水平上,其回收率在70%~100%范围内,批内、批间的相对标准偏差小于20%。A method for determination of triclabendazole and metabolite ketotriclabendazole in cattle tissues with HPLC by UV detection was established. Triclabendazole and ketotriclabendazole were extracted from cattle tissues with acetonitrile and degreased in isooctane,dissolved with 30% acidified alcohol after evaporation,then the solution was purified with PPL column. The mobile phase was 0. 02 mol / L ammonium acetate and acetonitrile,the flow rate was 1. 0 m L / min and detection wavelengths was 296 nm. A good linearity of the calibration curve was obtained with the range of 20 ~ 1200 ng / m L（ R2 0. 999）,and the limit of quantification of triclabendazole and ketotriclabendazole was 50 μg / kg. The average recoveries of triclabendazole and ketotriclabendazole from spiked cattle tissues at the three concentrations of 50,300,600 μg / kg（ liver and kindey）; 50,200,400 μg / kg（ muscle）and 50,100,200 μg / kg（ fat） ranged from 70% to 100%（ n = 5） respectively. In day- between days batch variation coefficients were both less than 20%.

关 键 词：三氯苯唑及其代谢物 残留 牛组织 高效液相色谱法 

分 类 号：S859.84[农业科学—临床兽医学]