采用寡糖CZE指纹图谱鉴定北沙参和南沙参  

Identification of Glehniae Radix and Adenophorae Radix by capillary zone electrophoresis fingerprints of oligosaccharides

作　　者：杨飞飞[1,2] 郭怀忠[1,2,3] 王晓欢[1] 代雅茹 王敬朝 高万丰 

机构地区：[1]河北大学药学院,河北保定071002 [2]河北省药物质量分析控制重点实验室,河北保定071002 [3]药物化学与分子诊断教育部重点实验室,河北保定071002

出　　处：《中南药学》2015年第5期455-457,共3页Central South Pharmacy

基　　金：国家自然科学基金(No.21011140338);河北大学2014年精品实验项目(No.SY201415)

摘　　要：目的利用中药多糖降解寡糖鉴别易混淆中药材北沙参和南沙参。方法采用水提醇沉法提取药材中的多糖,酸法控制降解多糖得到寡糖,1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生寡糖后采用毛细管区带电泳法(CZE)进行分离分析,电泳条件如下:未涂层熔融石英毛细管柱(70 cm×50μm,有效长度61 cm),以40 mmol·L-1硼砂缓冲溶液为运行缓冲液(p H=10.25),检测波长为245 nm,运行电压为18 k V,虹吸进样10 cm×8 s。利用得到的特征性寡糖指纹谱图,结合聚类分析(CA)鉴定北沙参和南沙参。结果采用本方法鉴定区分了不同来源的10批北沙参和南沙参药材。结论本方法可有效用于北沙参和南沙参的鉴定,结果可靠,重现性好,在中药材鉴定上具有良好的应用前景。Objective To identify the confusable traditional Chinese medicines （TCMs） of Glehniae Radix and Adenophorae Radix by degraded oligosaccharides from their polysaccharides. Methods Polysaceharides of the 2 TCMs were extracted by water extraction and alcohol precipitation method, and were degraded to oligosaccharides with acid. Then the PMP labeled oligosaccharides were analyzed by capillary zone electrophoresis （CZE）, the electrophoresis conditions were as follows： uncoated fused silica capillary column （70 em~ 50 ~tm, effective length 61 cm）, 40 mmol ~ L - ~borax solution （pH ----- 10.25） as the running buffer, the detection wavelength 245 nm; the operation voltage 18 kV, hydrodynamic pressure injection （10 cm X 8 s）. Glehniae Radix and Adenophorae Radix were identified by the featured oligosaccharide fingerprints assisted with cluster analysis. Results Ten batches of Glehniae Radix and Adenophorae Radix from different sources were identified successfully. Conclusion The method is reliable and reproducible, which can be used to effectively identify Glehniae Radix and Adenophorae Radix, and has a good application prospect in the identification of TCMs.

关 键 词：北沙参 南沙参 毛细管区带电泳法 聚类分析 寡糖 鉴定 

分 类 号：R284[医药卫生—中药学] O657.7[医药卫生—中医学]