钼暴露对雄性小鼠血清生化指标的影响  

Effect of Molybdenum Exposure on the Serum Biochemical Indexes of Male Mice

作　　者：李君[1] 柳晓琳[1] 吴鹏[1] 

机构地区：[1]辽宁医学院公共卫生学院,辽宁锦州121001

出　　处：《辽宁医学院学报》2015年第3期1-3,共3页Journal of Liaoning Medical University (LNMU) Bimonthly

摘　　要：目的探讨钼暴露对雄性小鼠机体血清生化指标的影响。方法选用50只35日龄健康雄性昆明小鼠,按体重随机分为5组,分别为对照组(生理盐水)、3.440、6.880、34.400、68.800 mg/kg钼酸钠染毒组,每组10只。以腹腔注射方式进行染毒,每日1次,连续染毒30 d,实验过程中准确观察小鼠日常行为及体重变化情况,染毒结束后,断头取血,测定染毒动物血清中谷丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性、乳酸脱氢酶(LDH)以及肌酐(Cr)、尿素氮(BUN)、尿酸(UA)含量。结果随着染毒剂量的增加,小鼠的体重增长缓慢,34.400、68.800 mg/kg染毒组小鼠体重增加同对照组比较差异有统计学意义(P<0.05)。血清中ALT、AST、LDH活性也随着染毒剂量的增加呈上升趋势,68.800 mg/kg染毒组ALT、AST、LDH活性同对照组比较差异有统计学意义,34.400 mg/kg染毒组ALT活性同对照组相比有统计学意义。Cr、BUN、UA的含量均升高,其中34.400、68.800 mg/kg染毒组同对照组比较差异有统计学意义。结论高剂量的钼可抑制雄性小鼠生长,并对小鼠血清生化指标ALT、AST、LDH、Cr、BUN、UA存在影响,提示雄性小鼠钼中毒后肝脏、心脏、肾脏可能受到不同程度的损伤。Objective To investigate the effect of molybdenum exposure on the serum biochemical indexes of male mice. Meth- ods A total of 50 male Kunming mice were selected and randomly divided into 5 groups based on their weight, with 10 mice in each group. Apart from control group （physiological saline）, the other four groups were established according to the dosage of sodium mo- lybdate exposure, 3. 440 mg/kg group, 6. 880 mg/kg group, 34. 400 mg/kg group, and 68. 800 mg/kg group. All the mice had been exposed through daily intraperitoneal injection successively for 30 days. During the 30 days, their daily behavior and weight change were observed. After the exposure, the AST, ALT, LDH, Cr, BUN and UA in the serum samples collected from those cut - head mice were measured. Results With the increase of exposure dosage, the weight gain of these mice remained slow. The difference in the weight gain between 34. 400 mg/kg and 68. 800 mg/kg groups and the control group was statistically significant （ P 〈 0. 05 ）. The activity of ALT, AST and LDH in the serum enhanced with the increase of exposure dosage, and the difference between 68. 800 mg/kg group and the control group was statistically significant, so was the difference in the activity of ALT between 34. 400 mg/kg group and the control group. The content of Cr, BUN, and UA in all the groups rose. The difference in the rise between 34. 400 mg/kg and 68. 800 mg/kg groups and the control group was statistically significant. Conclusion High dosage of molybdenum exposure can inhibit the growth of male mice and influence the serum biochemical indexes of ALT, AST, LDH, Cr, BUN and UA, which suggests that molybdenum poisoning may bring damage of different degrees to the liver, heart and kidney.

关 键 词：小鼠 钼暴露 生化指标 

分 类 号：Q555[生物学—生物化学]