黄芩苷对帕金森病小鼠模型的影响  

Effects of Baicalin on mouse model with Parkinson's disease

作　　者：吴纪凯[1] 王琳[1] 张轶[1] 梁睿[1] 

机构地区：[1]苏州卫生职业技术学院,江苏苏州215000

出　　处：《长春中医药大学学报》2015年第3期454-456,共3页Journal of Changchun University of Chinese Medicine

基　　金：苏州市吴门医学发展中心基金项目"吴门民间验方的现代制剂研究"(201412)

摘　　要：目的研究黄芩苷对帕金森病小鼠模型的影响。方法 60只雄性C57BL小鼠随机分为模型组、黄芩苷组、美多巴组、正常对照组。模型组小鼠腹腔注射30 mg/kg MPTP;黄芩苷组分别给予100 mg/kg黄芩苷灌胃和腹腔注射30 mg/kg MPTP;美多巴组给予31 mg/kg美多巴灌胃,1次/d;正常组给予等量生理盐水,1次/d,15 d。对4组小鼠的运动功能、脑纹状体DA、脑组织GSH、GSH-Px、MDA含量进行测定和统计分析。结果模型组小鼠的悬挂实验、游泳实验分值均明显比黄芩苷组、美多巴组和正常对照组高(P<0.05);黄芩苷组的脑纹状体DA水平、GSH水平均明显比美多巴组和模型组高(P<0.05);黄芩苷组小鼠的脑纹状体GSHPx、MDA水平均显著低于美多巴组和模型组(P<0.05)。结论黄芩苷预防给药能够有效保护帕金森病小鼠的神经功能,其作用机制可能与促进脑内抗氧化物质GSH水平的有效提升有相关联系。Objective To study the effects of baicalin on mouse model with Parkinson＇s disease. Methods The 60 male C57BL mice were were randomly divided into model group, baicalin group, the US-dopa group and normal control group, the model group were given intraperitoneal injection 30 mg/kgMPTP, the baicalin group were given 100 mg/kg baicalin gavage 30 mg/kgMPTP, the US-dopa group to 31 mg/kg lavage treatment, 1 times a day, the normal group was given equal saline, 1 times a day, 15 d. Then the motor function, brain striatal DA, brain tissue GSH, GSH-Px, MDA content and nigra TH im- munohistochemical staining of the three groups of mice were measured and statistically analyzed. Results Model mice sus- pension test, swimming test scores were significantly higher than the baicalin group, US-dopa group and normal control group （ P 〈 0.05） ; brain striatal DA levels baicalin group, GSH levels were significantly Bimei dopa and model groups of high （ P 〈 0.05） ; striatum baicalin group of mice GSH-Px, MDA levels were significantly lower than madopar group and model group （ P 〈 0.05）. Conclusion Baicalin prophylaxis can effectively protect mice with Parkinson＇s disease.

关 键 词：黄芩苷 帕金森病小鼠模型 运动功能 

分 类 号：R285.5[医药卫生—中药学]