橙皮苷抑制JNK信号通路辅助治疗2型糖尿病大鼠  

Hesperidin reduced insulin resistance index of diabetes rat by inhibiting JNK signaling pathway

作　　者：殷章红[1] 罗鹏程[1] 王才英[1] 

机构地区：[1]黄石市中心医院肾内科,湖北黄石435000

出　　处：《长春中医药大学学报》2015年第3期460-462,共3页Journal of Changchun University of Chinese Medicine

基　　金：湖北省科技厅资助项目子课题"橙皮苷对糖尿病肾病大鼠的治疗作用研究"(2012FFA016)

摘　　要：目的探讨橙皮苷治疗2型糖尿病大鼠后,其肝脏JNK信号通路3个关键分子JNK、P-JNK、Caspase-6表达的特点。方法 40只雄性SD大鼠,随机分为空白组(12只),模型组(14只),橙皮苷治疗组(14只),模型组和橙皮苷治疗组高脂饮食6个月形成2型糖尿病模型,治疗8周后收集肝脏行免疫组化检测JNK、P-JNK、Caspase-6分布及表达;Western blot及RT-PCR测定3个蛋白和mRNA表达。结果免疫组化结果示橙皮苷治疗组肝细胞相应蛋白表达JNK、P-JNK、Caspase-6较模型组减少(P<0.05),但和空白组差距仍然较大(P<0.05);JNK、P-JNK、Caspase-6蛋白以及mRNA表达,橙皮苷组低于模型组(P<0.05),但和对照组仍有一定的差异(P<0.05),胰岛素抵抗指数与上述3个蛋白改变趋势一致。结论橙皮苷可降低2型糖尿病胰岛素抵抗指数,其机制可能是通过抑制JNK信号通路中JNK、p-JNK以及Caspase-6的表达实现的。Objective Analysis liver JNK signaling pathway of diabetic after hesperidin treatment of SD rats. Methods With high fat fed into a insulin resistance mold, After the modeling 40 rats were randomly divided into a control group （12）, model group （lg）, hesperidin intervention group （14）, after treatment 8 weeks detected immunohistochemical detection asso- ciated protein expression;while Western and RT-PCR to detect the expression of the three proteins and mRNA of the liver. Results Pathological changes JNK, P-Jun, Caspase-6 significantly reduced compared with the model group of liver cells af- ter hesperidin treatment, However, compared with control group is still large;JNK, P-JNK, Caspase-6 protein and mRNA ex- pression, hesperidin group better than the untreated group （ P 〈 0.05）, and compared with the control group, still had some differences （ P 〈 0.05）. Conclusion Hesperidin can reducing JNK, p-JNK, Caspase-6 expression, reduce insulin resis- tance.

关 键 词：JNK P-JNK CASPASE-6 2型糖尿病 橙皮苷 

分 类 号：R285.5[医药卫生—中药学]