竞争性ELISA、HPLC测定清燥救肺汤中甘草酸的含量  

Competitive ELISA and HPLC in determination of glycyrrhizic acid in Qingzaojiufei decoction

作　　者：吴振起[1] 王思源[1] 平静[1] 于艳[2] 南春红[1] 

机构地区：[1]辽宁中医药大学附属医院儿科,沈阳110032 [2]辽宁中医药大学附属医院中药实验室,沈阳110032

出　　处：《长春中医药大学学报》2015年第3期478-481,共4页Journal of Changchun University of Chinese Medicine

基　　金：国家自然科学基金面上项目(81373687);辽宁省教育厅优秀人才基金(LJQ2011102);沈阳市科技基金(F11-264-1-62)

摘　　要：目的建立清燥救肺汤中甘草酸（GA）快速灵敏的免疫分析检测方法。方法以制备出的甘草酸特异性单克隆抗体为基础,选择单抗最佳工作浓度,建立GA间接竞争酶联免疫分析方法（ELISA）,并应用此方法检测清燥救肺汤中的GA含量。同时结合高效液相色谱法（HPLC）进行比较确证。结果 ELISA测定清燥救肺汤中成分GA在0.312 5~10μg/m L（r=0.996 9）范围内线性关系良好,平均回收率不低于92.5%;3批样品中GA含量均值为10.125 mg/g,与HPLC测定结果相近,RSD〈1%。结论 ELISA对中药成分测定准确可靠,重复性好,是HPLC测定有益的技术补充。Objective To establish an immunoassay method for the detennination of glycyrrhizic acid （GA） in Qingzaojiufei Tang. Methods Indirect competitive ELISA was developed by using anti-GA monoclonal antibody （anti-GA MAD）, and then it was applied to GA measurement in the traditional Chinese medicine Qingzaojiufei Tang. Simultaneously, using analy- sis method of HPLC confirm. Results GA had good linearity in the ranges of 0.312 5 - 10 μg/mL（r = 0.996 9）, the aver- age recoveries not less than 92.5 %, respectively. The average content of GA in three sample was 10. 125 mg/g by using ELISA. The RSD of GA determinations between ELISA and HPLC less than 1%, showed a high similarity. Conclusion The developed ELISA method is accurate with high repeatability, which is helpful to provides useful technical supplement for the determination of HPLC.

关 键 词：清燥救肺汤 甘草酸 ELISA HPLC 含量测定 

分 类 号：R284.2[医药卫生—中药学]