金莲花中黄酮的提纯研究  

Purification Research of Flavone from Trollius

作　　者：崔艳伟 王新 徐鹏[2] 

机构地区：[1]黑龙江完达山林海液奶有限公司,哈尔滨150078 [2]黑龙江科技大学机械工程学院,哈尔滨150027

出　　处：《中国奶牛》2015年第7期43-46,共4页China Dairy Cattle

摘　　要：本文采用微波法提取金莲花中黄酮,用大孔树脂对浸提液进行了提纯研究。微波法以微波时间、微波功率、固液比为三因素,设计正交试验,确定了最佳提取条件为:微波时间8min,微波功率200W,固液比1:20。提纯过程中用AB-8大孔树脂进行提纯,洗脱以乙醇浓度、乙醇用量、温度为三因素设计正交试验,确定了最佳提纯条件为:乙醇浓度75%,乙醇用量10倍,温度35℃。洗脱率可达91.6%,总黄酮纯度为51.2%。The microwave method for the extraction flavone from trollius, purification the extracting liquid by macro-reticular resin was studied in this paper. The wavelet time, wavelet power, solid-liquid tatio were regarded as three factors, by orthogonal experiment,the optimum extracting condition of lfavonoid from trollius was showed as follows：wavelet time was 8 minutes, wavelet power was 200W, solid –liquid ratio was 1：20.The AB-8 macro-reticular resin was used in purification process. The alcoholcon centration, quantity, temperature were regarded as three factors, by orthogonal experiment, the furthest purification condition was drawn as follows： alcoholcon centration was 75%, the ratio was 1：10, temperature was 35℃,thepurityoflfavonescanreachto51.2%.

关 键 词：金莲花 黄酮 大孔树脂 提取 纯化 

分 类 号：S858.23[农业科学—临床兽医学]