Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery  

作　　者：Li Li Wenyi Gu Jian Liu Shiyu Yan Zhi Ping Xu 

机构地区：[1]Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia [2]Department of Chemical Engineering, Curtin University, Perth, WA 6845, Australia

出　　处：《Nano Research》2015年第2期682-694,共13页纳米研究（英文版）

摘　　要：In this work a novel strategy has been developed to prepare well-dispersed amine-functionalized SiO2 nanodot-coated layered double hydroxide nano- composite （NH2-SiO2@LDH） via electrostatic interactions and condensation of （3-aminopropyl）triethoxysilane （APTES）. This nanocomposite system is well dispersed in culture media and phosphate buffered saline, and exhibits low cytotoxicity and good biocompatibility. The fluorescence microscopy images and flow cytometry data indicate that such an NH2-SiO2@LDH nanocomposite is able to efficiently deliver small interfering RNA （siRNA） into the U2OS cell line to inhibit cell proliferation. Thus, NH2-SiOR@LDH nanocomposite has a great potential as a nanocarrier for efficient gene delivery.在这个工作，新奇策略被开发了准备分散得好的 amine-functionalized SiO <潜水艇class=“ a-plus-plus ”> 2 </sub>nanodot涂的分层的双氢氧化物 nanocomposite ( NH <潜水艇class=“ a-plus-plus ”> 2 </sub>-SiO<sub class=“ a-plus-plus ”>经由静电的相互作用和冷凝作用的 2 </sub>@LDH)( 3-aminopropyl ) triethoxysilane ( APTES )。这个 nanocomposite 系统很好在缓冲的文化媒介和磷酸盐被驱散盐，并且展出低 cytotoxicity 和好 biocompatibility。荧光显微镜学图象和流动 cytometry 数据显示那如此的 NH < 潜水艇 class= “ a-plus-plus ” > 2 </sub>-SiO<sub class= “ a-plus-plus ” > 2 </sub>@LDH nanocomposite 能高效地交付小介入的 RNA (siRNA ) 进 U2OS 房间线禁止房间增长。因此， NH < 潜水艇 class= “ a-plus-plus ” > 2 </sub>-SiO<sub class= “ a-plus-plus ” > 2 </sub>@LDH nanocomposite 为有效基因交货作为 nanocarrier 有一个大潜力。

关 键 词：layered double hydroxide（LDH） FUNCTIONALIZATION self-assembl3~gene delivery 

分 类 号：TQ314[化学工程—高聚物工业] TB332[一般工业技术—材料科学与工程]