“大五星”天然三倍体枇杷株系的SSR分析  

Genetic Analysis of“Dawuxing”Natural Triploid Loquats Based on SSR

作　　者：徐波[1,2] 姜琬[1] 李晓林[1] 郭启高[1] 孙海艳[1] 向素琼[1] 何桥[1] 梁国鲁[1] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]重庆市巴南区农产品质量安全中心,重庆401320

出　　处：《西南大学学报（自然科学版）》2015年第3期22-27,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家科技支撑计划项目(2013BAD02B02)

摘　　要：利用从103对简单重复序列（SSR）引物中筛选出的62对多态性引物,对8份＂大五星＂天然三倍体枇杷株系和1份二倍体枇杷单株进行PCR扩增,共扩增出152个等位位点,每个位点扩增出2~3个等位基因,平均2.45个,引物多态信息量介于0.099至0.586之间,平均多态信息量为0.358.62对SSR引物将供试枇杷株系完全区别开,各株系间Dice相似系数范围为0.493~0.954,A376与A368株系之间的Dice相似系数最高,A313与A332株系之间的Dice相似系数最低.在62对引物中,CL22.Contig1,NZ02b01,Hi15h12,EJ014,CH04c06和CH01h02共6对引物能鉴定出A379,A322,A348和A332这4个＂大五星＂天然三倍体枇杷株系的倍性.Sixty-two high ers, amplified 152 alleles polymorphism pairs of SSR primers, screened out from 103 pairs of SSR prim- in 8 natural triploids and one diploid of ＇Dawuxing＇ loquat. The number of alleles per locus ranged from 2 to 3, and averaged 2.45. The value of allelic polymorphism information content （PIC）ranged from 0. 099 to 0. 586, and averaged 0. 358. The 9 loquat trees could be differentiated com- pletely by the 62 pairs SSR primers. The Dice similarity coefficient among the trees varied from 0. 493 to 0. 954, the maximum was detected between A376 and A368 and the minimum between A313 and A332. Of the 62 pairs of primes, 6 pairs （CL22. Contigl, NZ02b01, Hi15h12, EJ014, CH04c06 and CH01h02） could identify the ploidy of A379, A322, A348 and A332.

关 键 词：枇杷 三倍体 简单重复序列(SSR) 

分 类 号：S667.3[农业科学—果树学]