头功铁药散对A型魏氏梭菌α毒素的mRNA表达及损伤小鼠免疫功能的影响  

Effects of Tougong Tieyao Powder on the Gene Expression ofα-Toxin mRNA and the Immunity Dysfunction of Mice Affected byα-Toxin of Clostridium welchii Type A

作　　者：赖建彬[1] 彭诗[1] 刘娟[1] 朱兆荣[1] 郭志兴[1] 

机构地区：[1]西南大学(荣昌校区)动物医学系,重庆荣昌402460

出　　处：《西南大学学报（自然科学版）》2015年第3期65-70,共6页Journal of Southwest University(Natural Science Edition)

基　　金：农业部国家公益行业(农业)科技专项金费(20130304-05)

摘　　要：探讨自拟处方头功铁药散对A型魏氏梭菌α-毒素损伤小鼠免疫功能的影响,并检测了α毒素mRNA表达的情况.检测小鼠部分免疫功能;荧光定量RT-PCR法检测α毒素基因mRNA表达的变化.结果显示,与模型组相比,头功铁药散能拮抗α-毒素对小鼠的损伤,极显著提高小鼠免疫器官指数及巨噬细胞的吞噬能力(p<0.01);与细菌模型组相比,头功铁药散能极显著降低α毒素基因表达(p<0.01).推测头功铁药散可通过提高小鼠免疫功能,并下调α毒素基因表达,从而减少α-毒素对小鼠的损伤.In a study reported in this paper, the effects of TouGong TieYao Powder, a medicine developed by the authors, on the immune function of mice injured by the exotosin of Clostriclium welchii Type A were investigated and the expression of a toxin mRNA was detected with fluorescence quantitative RT- PCR. Compared with the model group, Tougong Tieyao Powder helped to antagonize the injure caused by exotosin of C. vaelchii and improve the weight index of spleen and thymus, and highly significantly （P〈 0.01） enhanced the phagocytic function of macrophages. Compared with the bacterial group, Tougong Tieyao Powder highly significantly （P〈0.01） decreased the gene expression of α-toxin. Conclusion. To- ugong Tieyao Powder may improve the immune function of mice by decreasing the expression of α toxin gene and reducing the injure by exotosin of C, welchii.

关 键 词：头功铁药散 A型魏氏梭菌 免疫功能 Α毒素 

分 类 号：S851.3[农业科学—预防兽医学]