Tracking morphologies at the nanoscale： Self-assembly of an amphiphilic designer peptide into a double helix superstructure  

作　　者：Karin Kornmueller Ilse Letofsky-Papst Kerstin Gradauer Christian Mikl Fernando Cacho-Nerin Mario Leypold Walter Keller Gerd Leitinger Heinz Amenitsch Ruth Prassl 

机构地区：[1]Institute of Biophysics, Medical University ofGraz, BioTechMed-Graz, 8010 Graz, Austria [2]Institute for Electron Microscopy and Nanoanalysis, Graz University of Technology and Graz Centre for Electron Microscopy, 80 10 Graz, A us tria [3]Institute of Inorganic Chemistry, Graz University of Technology, 8010 Graz, Austria [4]Institute of Organic Chemistry, Graz University of Technology, 8010 Graz, Austria [5]Institute for Molecular Biosciences, KarI-Franzens-University Graz, 8010 Graz, Austria [6]Research Unit Electron Microscopic Techniques, Institute of Cell Biology, Histology and Embryology, Medical University of Graz, 80 10 Graz, A us tria [7]Center for Medical Research, Medical University of Graz, 8010 Graz, Austria

出　　处：《Nano Research》2015年第6期1822-1833,共12页纳米研究（英文版）

摘　　要：Hierarchical self-assembly is a fundamental principle in nature, which gives rise to astonishing supramolecular architectures that are an inspiration for the development of innovative materials in nanotechnolog）a Here, we present the unique structure of a cone-shaped amphiphilic designer peptide. While tracking its concentration-dependent morphologies, we observed elongated bilayered single tapes at the beginning of the assembly process, which further developed into novel double-helix-like superstructures at high concentrations. This architecture is characterized by a tight intertwisting of two individual helices, resulting in a periodic pitch size over their total lengths of several hundred nanometers. Solution X-ray scattering data revealed a marked 2-layered internal organization. All these characteristics remained unaltered for the investigated period of almost three months. In their collective morphology, the assemblies are integrated into a network with hydrogel characteristics. Such a peptide-based structure holds promise as a building block for next-generation nanostructured biomaterials.层次自己组装是在自然的一个基本原则，它产生是为在纳米技术的创新材料的开发的灵感的令人震惊的超分子的体系结构。这里，我们在场锥形的 amphiphilic 设计者肽的唯一的结构。当追踪它的集中依赖者形态学时，我们在集会过程的开始观察了伸长的 bilayered 单身者磁带，它进一步在高集中发展成新奇 double-helix-like 上层建筑。这体系结构被二单个 helices 的紧密的 intertwisting 描绘，导致在他们几百纳米的全部的长度上的一种周期的沥青尺寸。解决方案 X 光检查散布数据揭示了显著 2-layered 内部组织。所有这些特征为几乎三个月的调查时期仍然保持未改变。在他们的集体形态学，集会集成于有 hydrogel 特征的一个网络。如此的基于肽的结构为下一代的 nanostructured biomaterials 作为一块积木保持诺言。

关 键 词：double helix amphiphilic designerpeptide self-assembl）~SAXS TEM spectroscopy 

分 类 号：TB383[一般工业技术—材料科学与工程] Q523[生物学—生物化学]