姜黄素预处理保护急性脊髓损伤小鼠神经功能的实验研究  

Experimental study of the improvement on neurologic function of curcumin preconditioning on acute spinal cord injury in mice

作　　者：张南文[1] 叶剑[2] 蔡燕亮 何云娇[3] 王金填 陈建清[3] 吴增楷 魏桂财[2] 

机构地区：[1]福建医科大学药学院,福州350108 [2]南平市第一医院骨科,福建南平353000 [3]福建医科大学基础医学院,福州350108

出　　处：《中国临床药理学杂志》2015年第11期950-953,共4页The Chinese Journal of Clinical Pharmacology

基　　金：福建省卫生厅青年科研课题基金资助项目(2013-2-147);福建医科大学基础医学院大学生创新创业训练计划基金资助项目(2014JC006)

摘　　要：目的探讨姜黄素预处理对小鼠急性脊髓损伤后神经功能的保护作用及其可能机制。方法小鼠随机分为4组,假手术组、模型组、对照组(甲强龙,40mg·kg-1)和实验组(姜黄素,200 mg·kg-1)。造模前30 min,分别腹腔注射相应药物,用改良的Allen's法建立急性脊髓损伤模型后,进行行为学评价,用Western blot法测定脊髓核因子E2相关因子2(Nrf2)蛋白表达,用酶联免疫吸附法(ELISA)测定血清肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的表达。结果与模型组比较,实验组小鼠的后肢功能及斜板实验评分显著增加,Nrf2和TNF-α表达均明显改善,IL-6表达在8 h减少,但之后的时间点无明显差别。结论预防性给予姜黄素,可以通过上调Nrf2、下调炎症因子对小鼠起到神经功能的保护作用。Objective To explore the effect and mechanism of curcumin on neuronal protective function in acute spinal cord model （ ASCI） mice. Methods Divide healthy and clean class mice into sham -operated group,model group, control group （ methylprednisolone, 40 mg · kg^-1 ） and treatment group （ curcumin 200 mg · kg^-1 ）.Each mice respectively received injection in intraperitoneal with drugs at 30 min before the sur-gery.Then, experimental animal models was established following im-proved Allen′s model.The behavior of the model mice was performed.The nuclear factor E2-related faetor 2 （ Nrf 2 ） antibody expression was examined with Western blot.And expression of tumor necrosis factor-α（ TNF-α） and interleukin-6 （ IL-6 ） were examined with enzyme-linked immunosorbent assay.Results Compared to model group, beha-vior score,neurologic function,the expression of Nrf 2 and TNF -αin treatment group achieved significant improvement.The IL-6 expression in treatment group only revealed weaker in 8 h, but no notable difference compared to model group after 8 h.Conclusion The preliminary study shows that the way of curcumin preconditioning to improve neurologic function may by decreasing inflammatory factor and raising the expression of Nrf2.

关 键 词：急性脊髓损伤 姜黄素 氧化应激 炎症 

分 类 号：R285.5[医药卫生—中药学] R965.1[医药卫生—中医学]