基于微乳薄层色谱技术分离鉴定2种医院制剂中的黄酮类成分  

Separation and Identification of Flavonoids from Two Kinds of Hospital Preparations by Micro-emulsion Thin Layer Chromatography

作　　者：高颖[1] 房德敏[1] 周波 严震 

机构地区：[1]天津市天津医院,天津300211 [2]重庆市合川区妇幼保健院,重庆401520 [3]梧州市新型农村合作医疗管理中心,广西梧州543000

出　　处：《中国实验方剂学杂志》2015年第12期58-61,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立舒筋定痛胶囊和苏氏接骨胶囊中黄酮类成分的微乳薄层色谱鉴别方法,为该技术的推广提供参考。方法:以微乳液为展开剂,聚酰胺薄膜为固定相,通过单因素试验考察表面活性剂的种类和浓度、助表面活性剂、油相、含水量及有机改性剂等对分离度的影响。通过调节微乳体系各组分的组成,优选薄层色谱条件分离检测舒筋定痛胶囊和苏氏接骨胶囊中黄酮类有效成分。结果:以含水量75%的十二烷基硫酸钠(SDS)-正丁醇-正辛烷(3.4∶7.3∶2.0)微乳液-甲酸(9∶1)为展开剂可分离鉴别出舒筋定痛胶囊中柚皮苷;以含水量75%的SDS-正丁醇-正庚烷(2.7∶6.3∶1.0)微乳液-甲酸(9∶1)为展开剂可分离鉴别出苏氏接骨胶囊中金丝桃苷。结论:建立的微乳薄层色谱方法简便、准确、高效,可用于苏氏接骨胶囊中菟丝子和舒筋定痛胶囊中骨碎补的定性鉴别。Objective： To investigate application of micro-emulsion thin layer chromatography （ME- TLC） in separation and identification of flavonoids in Shujin Dingtong capsules and Su＇s Jiegu capsules. Method： Using microemulsion as developing agent in combination with polyamide film as the stationary phase, factors for microemulsion resolution, such as types and concentrations of surfactants, co-surfactants, the oil phase, impact of moisture and organic modifier were investigated by adjusting composition of each component in microemulsion system to find the best TLC conditions for separation and detection of flavonoids in Shujin Dingtong capsules and Su＇s Jiegu capsules. Result： Naringin in Shujin Dingtong capsules were separated and identified simultaneously with developing agent of micro-emulsion form-acetic acid （9：1 ）, micro-emulsion was sodium dodecyl sulfate （SDS） -n-butyl alcohol-n-octane-H20 （3.4： 7.3： 2.0： 75.0）. Hyperin in Su＇s Jiegu capsules was separated and identified simultaneously with developing agent of micro-emulsion form-acetic acid （9：1 ）, micro-emulsion was SDS-n-butyl alcohol-n-heptane-H2O （2.7： 6.3： 1.0： 75.0）. Compared with the organic developing solvent, separation effect of micro-emulsion was improved. Conclusion： METLC is a new and efficient method for separation and identification of flavonoids in Shujin Dingtong capsules and Su＇s Jiegu capsules.

关 键 词：微乳液 薄层色谱 柚皮苷 金丝桃苷 舒筋定痛胶囊 苏氏接骨胶囊 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]