淫羊藿-女贞子抗维甲酸致大鼠骨质疏松症的作用机制探讨  

Experimental Study of Compatibility of Epimedii Folium and Ligustri Lucidi Fructus on Retinoic Acidinduced Osteoporosis in Rats

作　　者：李梅 康学[2] 年宏蕾 史浩田[2] 王秀娟[2] 刘仁慧[2] 

机构地区：[1]山东省莱阳卫生学校,山东莱阳265200 [2]首都医科大学中医药学院,北京100069

出　　处：《中国实验方剂学杂志》2015年第12期81-85,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81373814);国家中医药管理局"十二五"中医药教育教学改革研究课题(ZJYJY12011);北京市教育委员会科技计划面上项目(KM201310025012);北京中医药薪火传承"3+3"工程二室一站建设项目(2012-SZ-C-42)

摘　　要：目的:研究淫羊藿女贞子水煎剂及其有效成分提取物防治维甲酸(RA)致大鼠骨质疏松症(OP)的作用机制。方法:SPF级雄性Wistar大鼠36只,随机分为6组,即正常组、模型组、淫羊藿-女贞子水煎剂组、淫羊藿-女贞子提取物低、中、高剂量组,RA(70 mg·kg-1)ig造模,造模的同时给药,给药组分别给予淫羊藿-女贞子水煎剂(9.5 g·kg-1)及药对有效成分提取物低、中、高3个剂量(50,100,200 mg·kg-1)ig治疗2周,免疫组化法检测骨组织中凋亡相关因子B细胞淋巴瘤/白血病-2(Bcl-2),p53的蛋白表达;实时RT-q PCR法检测骨组织中转化生长因子-β1(TGF-β1),果蝇MAD类似基因2(Smad2),Smad3,Smad7 mRNA的表达。结果:与正常组比较,模型组大鼠骨组织Bcl-2蛋白表达明显降低,而p53蛋白表达显著升高,骨组织TGF-β1及Smad2,Smad3 mRNA相对表达量均明显下降,Smad7 mRNA相对表达量明显升高,均具有统计学差异(P<0.05,P<0.01);与模型组比较,各给药组可不同程度上调Bcl-2以及TGF-β1,Smad2,Smad3的表达水平,下调p53,Smad7的表达水平,均具有明显统计学差异(P<0.05,P<0.01)。结论:淫羊藿-女贞子抗OP的作用机制可能与其维持凋亡与抗凋亡间的平衡,抑制骨组织细胞凋亡以及调控TGF-β/Smads通路信号转导有关。Objective： To investigate the mechanism of the compatibility of Epimedii Folium and Ligustri Lucidi Fructus decoction and their extract on retinoic acid-induced osteoporosis in rats. Method： Thirty-six male Wistar rats were randomly divided into 6 groups： the normal group, the model group, the combination of Epimedii Folium and Ligustri Lucidi Fructus group （9.5 g·kg^-1） , and three extract combination groups （Extract- L 50 mg·kg^-1 , Extract-M 100 mg·kg^-1 , and Extract-H 200 mg·kg^-1 ） of 10 rats each. The osteoporosis model was induced in rats by intragastric administration of retinoic acid. The protein expressions of apoptosis factor B-cell lymphoma/Leukemia-2 （Bcl-2） and p53 of bone tissue were detected by immunohistochemical methods. The mRNA expressions of transforming growth factor beta-1 （TGF-/31）, Smad2, Smad3 and Smad7 of bone tissue were detected by using real-time RT-PCR. Result： Compared with the normal group, the protein expression of Bcl-2 decreased significantly, p53 protein increased significantly, mRNA expressions of TGF-±1, Smad2, Smad3 decreased significantly, and Smad7 increased significantly in the model group （P 〈 O. 05, P 〈 O. O1 ）. Compared with the model group, the expression levels of Bcl-2, TGF-β1, Smad2 and Smad3 increased, the expression levels of p53 and Smad7 decreased in all treatment groups （P 〈0.05, P 〈0.01 ）. Conclusion： The mechanism of the compatibility decoction and their extracts against osteoporosis is related to maintaining the balance between apoptosis and anti-apoptotic, inhibiting of apoptosis of bone tissue, regulating and controlling the TGF-β/Smads signal transduction pathways.

关 键 词：淫羊藿-女贞子 维甲酸 骨质疏松症 

分 类 号：R285.5[医药卫生—中药学]